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The predominant pathogenicity determinant produced by\n                    <jats:italic>S. scabies<\/jats:italic>\n                    is the thaxtomin A phytotoxin, which is essential for common scab disease development. Production of thaxtomin A involves the nonribosomal peptide synthetases (NRPSs) TxtA and TxtB, both of which contain an adenylation (A\u2010) domain for selecting and activating the appropriate amino acid during thaxtomin biosynthesis. The genome of\n                    <jats:italic>S. scabies<\/jats:italic>\n                    87.22 contains three small MbtH\u2010like protein (MLP)\u2010coding genes, one of which (\n                    <jats:italic>txtH<\/jats:italic>\n                    ) is present in the thaxtomin biosynthesis gene cluster. MLP family members are typically required for the proper folding of NRPS A\u2010domains and\/or stimulating their activities. This study investigated the importance of TxtH during thaxtomin biosynthesis in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    . Biochemical studies showed that TxtH is required for promoting the soluble expression of both the TxtA and TxtB A\u2010domains in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , and amino acid residues essential for this activity were identified. Deletion of\n                    <jats:italic>txtH<\/jats:italic>\n                    in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    significantly reduced thaxtomin A production, and deletion of one of the two additional MLP homologues in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    completely abolished production. Engineered expression of all three\n                    <jats:italic>S. scabies<\/jats:italic>\n                    MLPs could restore thaxtomin A production in a triple MLP\u2010deficient strain, while engineered expression of MLPs from other\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    spp. could not. Furthermore, the constructed MLP mutants were reduced in virulence compared to wild\u2010type\n                    <jats:italic>S. scabies.<\/jats:italic>\n                    The results of our study confirm that TxtH plays a key role in thaxtomin A biosynthesis and plant pathogenicity in\n                    <jats:italic>S. scabies.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1111\/mpp.12843","type":"journal-article","created":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T01:53:21Z","timestamp":1562550801000},"page":"1379-1393","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["TxtH is a key component of the thaxtomin biosynthetic machinery in the potato common scab pathogen\n                    <i>Streptomyces scabies<\/i>"],"prefix":"10.1111","volume":"20","author":[{"given":"Yuting","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biology Memorial University of Newfoundland 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