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However, the mechanism by which \u03b1\u2010MMC increases plant resistance to viral infections remains unclear. To study the effect of \u03b1\u2010MMC on plant viral defence and how \u03b1\u2010MMC increases plant resistance to viruses, recombinant DNA and transgenic technologies were employed to investigate the role of \u03b1\u2010MMC in <jats:italic>Nicotiana benthamiana<\/jats:italic> resistance to tobacco mosaic virus (TMV) infection. Treatment with \u03b1\u2010MMC produced through DNA recombinant technology or overexpression of \u03b1\u2010MMC mediated by transgenic technology alleviated TMV\u2010induced oxidative damage and reduced the accumulation of reactive oxygen species (ROS) during TMV\u2010green fluorescent protein infection of <jats:italic>N.\u00a0benthamiana<\/jats:italic>. There was a significant decrease in TMV replication in the upper leaves following local \u03b1\u2010MMC treatment and in <jats:italic>\u03b1\u2010MMC<\/jats:italic>\u2010overexpressing plants relative to control plants. These results suggest that application or overexpression of \u03b1\u2010MMC in <jats:italic>N.\u00a0benthamiana<\/jats:italic> increases resistance to TMV infection. Finally, our results showed that overexpression of \u03b1\u2010MMC up\u2010regulated the expression of ROS scavenging\u2010related genes. \u03b1\u2010MMC confers resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme\u2010encoding genes. Overall, our study revealed a new crosstalk mechanism between \u03b1\u2010MMC and ROS during resistance to viral infection and provides a framework to understand the molecular mechanisms of \u03b1\u2010MMC in plant defence against viral pathogens.<\/jats:p>","DOI":"10.1111\/mpp.12974","type":"journal-article","created":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T07:30:21Z","timestamp":1595748621000},"page":"1212-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Alpha\u2010momorcharin enhances <i>Nicotiana benthamiana<\/i> resistance to tobacco mosaic virus infection through modulation of reactive oxygen species"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6853-7291","authenticated-orcid":false,"given":"Feng","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Horticulture and Plant Protection Joint International Research Laboratory of 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