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However, little research has been done to understand the function of those <jats:italic>CSN<\/jats:italic> genes in broad\u2010spectrum resistance to pathogens. In this study, we found that the transcript levels of wheat <jats:italic>TaCSN5<\/jats:italic> were induced in response to inoculation with <jats:italic>Puccinia striiformis<\/jats:italic> f. sp. <jats:italic>tritici<\/jats:italic> (Pst) and treatment with salicylic acid (SA). Overexpression of <jats:italic>TaCSN5<\/jats:italic> in <jats:italic>Arabidopsis<\/jats:italic> resulted in increased susceptibility to <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato<\/jats:italic> DC3000 infection accompanied by down\u2010regulation of <jats:italic>AtPR1<\/jats:italic> expression. Overexpression of <jats:italic>TaCSN5<\/jats:italic> in wheat lines significantly increased susceptibility to Pst accompanied by decreased SA accumulation, whereas <jats:italic>TaCSN5<\/jats:italic>\u2010RNAi wheat lines exhibited opposite trends. Moreover, we found that <jats:italic>TaCSN5<\/jats:italic> negatively regulated <jats:italic>TaG3NPR1<\/jats:italic> genes involved in the SA signalling pathway. In addition, <jats:italic>TaCSN5<\/jats:italic>\u2010RNAi lines showed increased resistance to multiple races of Pst. Taken together, we demonstrate that <jats:italic>TaCSN5<\/jats:italic> contributes to negative regulation of wheat resistance to Pst in an SA\u2010dependent manner.<\/jats:p>","DOI":"10.1111\/mpp.13034","type":"journal-article","created":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T11:31:46Z","timestamp":1611487906000},"page":"410-421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["RNAi\u2010mediated stable silencing of <i>TaCSN5<\/i> confers broad\u2010spectrum resistance to <i>Puccinia striiformis<\/i> f. sp. <i>tritici<\/i>"],"prefix":"10.1111","volume":"22","author":[{"given":"Xingxuan","family":"Bai","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&amp;F University Yangling 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