{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:22:28Z","timestamp":1789179748886,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T00:00:00Z","timestamp":1623283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0100400"],"award-info":[{"award-number":["2017YFD0100400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31901959"],"award-info":[{"award-number":["31901959"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010014","name":"Six Talent Peaks Project in Jiangsu Province","doi-asserted-by":"publisher","award":["NY\u2010056"],"award-info":[{"award-number":["NY\u2010056"]}],"id":[{"id":"10.13039\/501100010014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    ARGONAUTE (AGO) proteins play crucial roles in plant defence against virus invasion. To date, the role of\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    in rice antiviral defence remains largely unknown. In this study, we determined that the expression of\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    in rice was induced upon rice black\u2010streaked dwarf virus (RBSDV) infection. Using transgenic rice plants overexpressing\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    and\n                    <jats:italic>Osago2<\/jats:italic>\n                    mutants generated through transposon\u2010insertion or CRISPR\/Cas9 technology, we found that overexpression of\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    enhanced rice susceptibility to RBSDV infection.\n                    <jats:italic>Osago2<\/jats:italic>\n                    mutant lines exhibited strong resistance to RBSDV infection through the elicitation of an early defence response, including reprogramming defence gene expression and production of reactive oxygen species (ROS). Compared to Nipponbare control, the expression level of\n                    <jats:italic>OsHXK1<\/jats:italic>\n                    (\n                    <jats:italic>HEXOKINASE 1<\/jats:italic>\n                    ) increased significantly, and the methylation levels of its promoter decreased in the\n                    <jats:italic>Osago2<\/jats:italic>\n                    mutant on RBSDV infection. The expression profile of\n                    <jats:italic>OsHXK1<\/jats:italic>\n                    was the opposite to that of\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    during RBSDV infection. Overexpression of\n                    <jats:italic>OsHXK1<\/jats:italic>\n                    in rice also induced ROS production and enhanced rice resistance to RBSDV infection. These results indicate that\n                    <jats:italic>OsHXK1<\/jats:italic>\n                    controls ROS accumulation and is regulated by\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    through epigenetic regulation. It is noteworthy that the\n                    <jats:italic>Osago2<\/jats:italic>\n                    mutant plants are also resistant to southern rice black\u2010streaked dwarf virus infection, another member of the genus\n                    <jats:italic>Fijivirus<\/jats:italic>\n                    . Based on the results presented in this paper, we conclude that\n                    <jats:italic>OsAGO2<\/jats:italic>\n                    modulates rice susceptibility to fijivirus infection by suppressing\n                    <jats:italic>OsHXK1<\/jats:italic>\n                    expression, leading to the onset of ROS\u2010mediated resistance. This discovery may benefit future rice breeding programmes for virus resistance.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13091","type":"journal-article","created":{"date-parts":[[2021,6,10]],"date-time":"2021-06-10T18:54:57Z","timestamp":1623351297000},"page":"1029-1040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["<i>ARGONAUTE 2<\/i>\n                    increases rice susceptibility to rice black\u2010streaked dwarf virus infection by epigenetically regulating\n                    <i>HEXOKINASE 1<\/i>\n                    expression"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5884-7982","authenticated-orcid":false,"given":"Zhaoyun","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyue","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"},{"name":"Department of Plant Pathology College of Plant Protection Nanjing Agricultural University Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"},{"name":"Key Laboratory of Agricultural Biodiversity and Disease Control of Ministry of Education College of Plant Protection Yunnan Agricultural University Kunming, Yunnan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"},{"name":"Department of Plant Pathology College of Plant Protection Nanjing Agricultural University Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejuan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Lan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjian","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-765X","authenticated-orcid":false,"given":"Hongwei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology College of Plant Protection Nanjing Agricultural University Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Food Quality and Safety Institute of Plant Protection Jiangsu Academy of Agricultural Sciences Nanjing, Jiangsu Province China"},{"name":"International Rice Research Institute and Jiangsu Academy of Agricultural Sciences Joint Laboratory Nanjing, Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,6,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a019471"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1171647"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16397"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00264"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00068"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2015.06.013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2011.04.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-18-0511"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006049"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209329109"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489453"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0039-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0071-x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014639"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.23"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2321"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.178012"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.10.009"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21165753"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.44.070505.143425"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-020-00382-8"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru447"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2015.04.007"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2011.03.007"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.02.051"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.205690"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1139\/o90-173"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05198"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805760105"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.178764"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(77)90502-5"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1102514"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2015.130"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13972"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2016.203"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05733"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13372"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.05.001"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.08.003"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00542"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.10.001"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-10-18-0285-R"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01665"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2016.49"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1817675116"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00270"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10509"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-12-16-1814-RE"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.13091","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mpp.13091","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.13091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T11:30:26Z","timestamp":1760959826000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.13091"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,10]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1111\/mpp.13091"],"URL":"https:\/\/doi.org\/10.1111\/mpp.13091","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,10]]},"assertion":[{"value":"2021-02-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}