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Here, we identified a putative endocytosis\u2010related gene, <jats:italic>PAL1<\/jats:italic>, which was highly expressed in appressorium of <jats:italic>Magnaporthe oryzae<\/jats:italic>, and was found to be important for appressorium formation and maturation. Deletion of <jats:italic>PAL1<\/jats:italic> significantly reduced the virulence of <jats:italic>M.\u00a0oryzae<\/jats:italic> due to defects in appressorial penetration and invasive growth in host cells. The Pal1 protein interacted and colocalized with the endocytosis protein Sla1, suggesting it is involved in endocytosis. The \u0394<jats:italic>pal1<\/jats:italic> mutant was significantly reduced in appressorium formation, which was recovered by adding exogenous cAMP and 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX). Moreover, the phosphorylation level of Pmk1 in \u0394<jats:italic>pal1<\/jats:italic> was also reduced, suggesting Pal1 functions upstream of both the cAMP and Pmk1 signalling pathways. As a consequence, the utilization of glycogen and lipid, appressorial autophagy, actin ring formation, localization of septin proteins, as well as turgor accumulation were all affected in the \u0394<jats:italic>pal1<\/jats:italic> mutant. Taken together, Pal1 regulates cAMP and the Pmk1 signalling pathway for appressorium formation and maturation to facilitate infection of <jats:italic>M.\u00a0oryzae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mpp.13149","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T14:55:40Z","timestamp":1634309740000},"page":"133-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of <i>Magnaporthe oryzae<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Deng","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center Changsha China"},{"name":"State Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province College of Plant Science and Technology Huazhong Agricultural 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