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Here, we characterized functional roles of a CysB regulator (RSc2427) in\n                    <jats:italic>R<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>solanacearum<\/jats:italic>\n                    OE1\u20101 that was demonstrated to be responsible for cysteine synthesis, expression of the T3SS genes, and pathogenicity of\n                    <jats:italic>R<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>solanacearum<\/jats:italic>\n                    . The\n                    <jats:italic>cysB<\/jats:italic>\n                    mutants were cysteine auxotrophs that failed to grow in minimal medium but grew slightly in host plants. Supplementary cysteine substantially restored the impaired growth of\n                    <jats:italic>cysB<\/jats:italic>\n                    mutants both in minimal medium and inside host plants. Genes of\n                    <jats:italic>cysU<\/jats:italic>\n                    and\n                    <jats:italic>cysI<\/jats:italic>\n                    regulons have been annotated to function for\n                    <jats:italic>R<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>solanacearum<\/jats:italic>\n                    cysteine synthesis; CysB positively regulated expression of these genes. Moreover, CysB positively regulated expression of the T3SS genes both in vitro and in planta through the PrhG to HrpB pathway, whilst impaired expression of the T3SS genes in\n                    <jats:italic>cysB<\/jats:italic>\n                    mutants was independent of growth deficiency under nutrient\u2010limited conditions. CysB was also demonstrated to be required for exopolysaccharide production and swimming motility, which contribute jointly to the host colonization and infection process of\n                    <jats:italic>R<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>solanacearum<\/jats:italic>\n                    . Thus, CysB was identified here as a novel regulator on the T3SS expression in\n                    <jats:italic>R.\u00a0solanacearum<\/jats:italic>\n                    . These results provide novel insights into understanding of various biological functions of CysB regulators and complex regulatory networks on the T3SS in\n                    <jats:italic>R.\u00a0solanacearum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mpp.13189","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T23:43:33Z","timestamp":1644018213000},"page":"679-692","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A CysB regulator positively regulates cysteine synthesis, expression of type III secretion system genes, and pathogenicity in\n                    <i>Ralstonia solanacearum<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"College of Resources and Environment Southwest University  Chongqing 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