{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:42:04Z","timestamp":1789756924899,"version":"4.0.1"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,4,17]],"date-time":"2022-04-17T00:00:00Z","timestamp":1650153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC31871914"],"award-info":[{"award-number":["NSFC31871914"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The initial stage of rice blast fungus,\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    , infection, before 36\u00a0h postinoculation, is a critical timespan for deploying pathogen effectors to overcome the host's defences and ultimately cause the disease. However, how this process is regulated at the transcription level remains largely unknown. This study functionally characterized two\n                    <jats:italic>M<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>oryzae<\/jats:italic>\n                    Early Infection\u2010induced Transcription Factor genes (\n                    <jats:italic>MOEITF1<\/jats:italic>\n                    and\n                    <jats:italic>MOEITF2<\/jats:italic>\n                    ) and analysed their roles in this process. Target gene deletion and mutant phenotype analysis showed that the mutants \u0394\n                    <jats:italic>moeitf1<\/jats:italic>\n                    and \u0394\n                    <jats:italic>moeitf2<\/jats:italic>\n                    were only defective for infection growth but not for vegetative growth, asexual\/sexual sporulation, conidial germination, and appressoria formation. Gene expression analysis of 30 putative effectors revealed that most effector genes were down\u2010regulated in mutants, implying a potential regulation by the transcription factors. Artificial overexpression of two severely down\u2010regulated effectors, T1REP and T2REP, in the mutants partially restored the pathogenicity of \u0394\n                    <jats:italic>moeitf1<\/jats:italic>\n                    and \u0394\n                    <jats:italic>moeitf2<\/jats:italic>\n                    , respectively, indicating that these are directly regulated. Yeast one\u2010hybrid assay and electrophoretic mobility shift assay indicated that Moeitf1 specifically bound the T1REP promoter and Moeitf2 specifically bound the T2REP promoter. Both T1REP and T2REP were predicted to be secreted during infection, and the mutants of T2REP were severely reduced in pathogenicity. Our results indicate crucial roles for the fungal\u2010specific Moeitf1 and Moeitf2 transcription factors in regulating an essential step in\n                    <jats:italic>M<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>oryzae<\/jats:italic>\n                    early establishment after penetrating rice epidermal cells, highlighting these as possible targets for disease control.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13224","type":"journal-article","created":{"date-parts":[[2022,4,17]],"date-time":"2022-04-17T13:58:32Z","timestamp":1650203912000},"page":"1200-1213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of two infection\u2010induced transcription factors of\n                    <i>Magnaporthe oryzae<\/i>\n                    reveals their roles in regulating early infection and effector expression"],"prefix":"10.1111","volume":"23","author":[{"given":"Yiyang","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuze","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghua","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and 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