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The appressorium, a specialized dome\u2010shaped infection structure, plays a crucial role in the pathogenesis of\n                    <jats:italic>C.\u00a0gloeosporioides<\/jats:italic>\n                    . However, the mechanism of how actin cytoskeleton dynamics regulate appressorium formation and penetration remains poorly defined in\n                    <jats:italic>C.\u00a0gloeosporioides<\/jats:italic>\n                    . In this study, an actin cross\u2010linking protein fimbrin homologue (CgFim1) was identified in\n                    <jats:italic>C.\u00a0gloeosporioides<\/jats:italic>\n                    , and the knockout of\n                    <jats:italic>CgFim1<\/jats:italic>\n                    led to impairment in vegetative growth, conidiation, and pathogenicity. We then investigated the roles of CgFim1 in the dynamic organization of the actin cytoskeleton. We observed that actin patches and cables localized at the apical and subapical regions of the hyphal tip, and showed a disc\u2010to\u2010ring dynamic around the pore during appressorium development. CgFim1 showed a similar distribution pattern to the actin cytoskeleton. Moreover, knockout of\n                    <jats:italic>CgFim1<\/jats:italic>\n                    affected the polarity of the actin cytoskeleton in the hyphal tip and disrupted the actin dynamics and ring structure formation in the appressorium, which prevented polar growth and appressorium development. The\n                    <jats:italic>CgFim1<\/jats:italic>\n                    mutant also interfered with the septin structure formation. This caused defects in pore wall overlay formation, pore contraction, and the extension of the penetration peg. These results reveal the mechanism by which CgFim1 regulates the growth and pathogenicity of\n                    <jats:italic>C.\u00a0gloeosporioides<\/jats:italic>\n                    by organizing the actin cytoskeleton.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13242","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T23:49:47Z","timestamp":1657064987000},"page":"1472-1486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Actin\u2010bundling protein fimbrin regulates pathogenicity via organizing F\u2010actin dynamics during appressorium development in\n                    <i>Colletotrichum gloeosporioides<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Yi","family":"Zhang","sequence":"first","affiliation":[{"name":"Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Key Laboratory of Biotechnology of Salt Tolerant Crops of Hainan Province College of Tropical Crops, Hainan University  Haikou China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang","family":"An","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Key Laboratory of Biotechnology of Salt Tolerant Crops of Hainan Province College of Tropical Crops, Hainan University  Haikou China"},{"name":"Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Key Laboratory of Biotechnology of Salt Tolerant Crops of Hainan Province College of Tropical Crops, Hainan University  Haikou 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