{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:47:39Z","timestamp":1790646459416,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["2017H1D3A1A01054585"],"award-info":[{"award-number":["2017H1D3A1A01054585"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infection of viruses from the genera\n                    <jats:italic>Bromovirus, Potyvirus<\/jats:italic>\n                    , and\n                    <jats:italic>Potexvirus<\/jats:italic>\n                    in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    induces significant up\u2010regulation of the genes that encode the HSP70 family, including binding immunoglobulin protein 2 (BiP2). Three up\u2010regulated genes were knocked down and infection assays with these knockdown lines demonstrated the importance of the\n                    <jats:italic>BiP2<\/jats:italic>\n                    gene for potyvirus infection but not for infection by the other tested viruses. Distinct symptoms of cucumber mosaic virus (CMV) and potato virus X (PVX) were observed in the\n                    <jats:italic>BiP2<\/jats:italic>\n                    knockdown line at 10\u2009days postagroinfiltration. Interestingly, following inoculation with either soybean mosaic virus (SMV) or pepper mottle virus (PepMoV) co\u2010expressing green fluorescent protein (GFP), neither crinkle symptoms nor GFP signals were observed in the\n                    <jats:italic>BiP2<\/jats:italic>\n                    knockdown line. Subsequent reverse transcription\u2010quantitative PCR analysis demonstrated that knockdown of\n                    <jats:italic>BiP2<\/jats:italic>\n                    resulted in a significant decrease of SMV and PepMoV RNA accumulation but not PVX or CMV RNA accumulation. Further yeast two\u2010hybrid and co\u2010immunoprecipitation analyses validated the interaction between BiP2 and nuclear inclusion protein b (NIb) of SMV\n                    <jats:italic>.<\/jats:italic>\n                    Together, our findings suggest the crucial role of BiP2 as a proviral host factor necessary for potyvirus infection. The interaction between BiP2 and NIb may be the critical factor determining susceptibility in\n                    <jats:italic>N.\u00a0benthamiana<\/jats:italic>\n                    , but further studies are needed to elucidate the underlying mechanism.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13284","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T04:36:12Z","timestamp":1669178172000},"page":"179-187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Binding immunoglobulin 2 functions as a proviral factor for potyvirus infections in\n                    <i>Nicotiana benthamiana<\/i>"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1033-734X","authenticated-orcid":false,"given":"Kristin","family":"Widyasari","sequence":"first","affiliation":[{"name":"Department of Agricultural Biotechnology Seoul National University  Seoul South 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