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The pathogenicity of\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    relies on the production of the secondary metabolite thaxtomin A. Little is known about the molecular mechanisms underlying the regulation of thaxtomin biosynthesis in\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    beyond the pathway\u2010specific activator TxtR and the cellulose utilization repressor CebR. The leucine\u2010responsive regulatory protein (Lrp) family modulates secondary metabolism in nonpathogenic streptomycetes. However, the regulatory relationship between the Lrp and pathogenic streptomycetes remains unknown. In this study, we demonstrated that SCAB_Lrp (SCAB_77931) from\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    significantly affects thaxtomin biosynthesis, pathogenicity, and morphological development.\n                    <jats:italic>SCAB_Lrp<\/jats:italic>\n                    deletion resulted in a dramatic decline in thaxtomin A production and a low\u2010virulence phenotype of\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    . An in\u2010depth dissection of the regulatory mechanism of SCAB_Lrp revealed that it positively regulates the transcription of the thaxtomin biosynthetic gene cluster by directly binding to the promoter of the cluster\u2010situated regulator gene\n                    <jats:italic>txtR<\/jats:italic>\n                    . SCAB_Lrp also controls the morphological development of\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    by directly activating the transcription of\n                    <jats:italic>amfC<\/jats:italic>\n                    ,\n                    <jats:italic>whiB<\/jats:italic>\n                    , and\n                    <jats:italic>ssgB<\/jats:italic>\n                    . SCAB_Lrp directly controls the transcription of its own gene by binding a specific sequence (5\u2032\u2010GGACAGTCGCCGTGCTACG\u20103\u2032). Moreover, phenylalanine and methionine have been characterized as SCAB_Lrp effectors by strengthening the binding affinity and complex status between SCAB_Lrp and DNA. Our findings characterize a multifunctional regulatory protein, SCAB_Lrp, that controls secondary metabolism, pathogenicity, and sporulation in\n                    <jats:italic>S.\u00a0scabies<\/jats:italic>\n                    and provide new insights into the complex regulatory network that modulates thaxtomin phytotoxins in pathogenic\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mpp.13285","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T13:21:26Z","timestamp":1670419286000},"page":"167-178","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The leucine\u2010responsive regulatory protein SCAB_Lrp modulates thaxtomin biosynthesis, pathogenicity, and morphological development in\n                    <i>Streptomyces scabies<\/i>"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3213-2978","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxia","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"He","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei 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