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Previous work suggested that invasive growth in this pathogen is controlled by environmental cues such as pH and nutrient status. Here we investigated the role of Target Of Rapamycin Complex 1 (TORC1), a global regulator of eukaryotic cell growth and development. Inactivation of the negative regulator Tuberous Sclerosis Complex 2 (Tsc2), but not constitutive activation of the positive regulator Gtr1, in\n                    <jats:italic>F.\u00a0oxysporum<\/jats:italic>\n                    resulted in inappropriate activation of TORC1 signalling under nutrient\u2010limiting conditions. The\n                    <jats:italic>tsc2<\/jats:italic>\n                    \u0394 mutants showed reduced colony growth on minimal medium with different nitrogen sources and increased sensitivity to cell wall or high temperature stress. Furthermore, these mutants were impaired in invasive hyphal growth across cellophane membranes and exhibited a marked decrease in virulence, both on tomato plants and on the invertebrate animal host\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    . Importantly, invasive hyphal growth in\n                    <jats:italic>tsc2<\/jats:italic>\n                    \u0394 strains was rescued by rapamycin\u2010mediated inhibition of TORC1. Collectively, these results reveal a key role of TORC1 signalling in the development and pathogenicity of\n                    <jats:italic>F.\u00a0oxysporum<\/jats:italic>\n                    and suggest new potential targets for controlling fungal infections.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13292","type":"journal-article","created":{"date-parts":[[2023,2,25]],"date-time":"2023-02-25T01:40:57Z","timestamp":1677289257000},"page":"289-301","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Constitutive activation of TORC1 signalling attenuates virulence in the cross\u2010kingdom fungal pathogen\n                    <i>Fusarium oxysporum<\/i>"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-0052","authenticated-orcid":false,"given":"Gesabel Yaneth","family":"Navarro\u2010Velasco","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica 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