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It utilizes exogenously applied double\u2010stranded RNA to specifically reduce pest target gene expression using endogenous RNA interference machinery. In this study, SIGS methods were developed and optimized for powdery mildew fungi, which are widespread obligate biotrophic fungi that infect agricultural crops, using the known azole\u2010fungicide target\n                    <jats:italic>cytochrome P450<\/jats:italic>\n                    51 (CYP51) in the\n                    <jats:italic>Golovinomyces orontii\u2013Arabidopsis thaliana<\/jats:italic>\n                    pathosystem. Additional screening resulted in the identification of conserved gene targets and processes important to powdery mildew proliferation:\n                    <jats:italic>apoptosis\u2010antagonizing transcription factor<\/jats:italic>\n                    in essential cellular metabolism and stress response; lipid catabolism genes\n                    <jats:italic>lipase a<\/jats:italic>\n                    ,\n                    <jats:italic>lipase 1<\/jats:italic>\n                    , and\n                    <jats:italic>acetyl\u2010CoA oxidase<\/jats:italic>\n                    in energy production\n                    <jats:italic>;<\/jats:italic>\n                    and genes involved in manipulation of the plant host via abscisic acid metabolism (\n                    <jats:italic>9\u2010cis\u2010epoxycarotenoid dioxygenase<\/jats:italic>\n                    ,\n                    <jats:italic>xanthoxin dehydrogenase<\/jats:italic>\n                    , and a putative\n                    <jats:italic>abscisic acid G\u2010protein coupled receptor<\/jats:italic>\n                    ) and secretion of the effector protein,\n                    <jats:italic>effector candidate 2<\/jats:italic>\n                    . Powdery mildew is the dominant disease impacting grapes and extensive powdery mildew resistance to applied fungicides has been reported. We therefore developed SIGS for the\n                    <jats:italic>Erysiphe necator\u2013Vitis vinifera<\/jats:italic>\n                    system and tested six successful targets identified using the\n                    <jats:italic>G.\u2009orontii\u2013A.\u2009thaliana<\/jats:italic>\n                    system. For all targets tested, a similar reduction in powdery mildew disease was observed between systems. This indicates screening of broadly conserved targets in the\n                    <jats:italic>G.\u2009orontii\u2013A.\u2009thaliana<\/jats:italic>\n                    pathosystem identifies targets and processes for the successful control of other powdery mildew fungi. The efficacy of SIGS on powdery mildew fungi makes SIGS an exciting prospect for commercial powdery mildew control.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13361","type":"journal-article","created":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:54:12Z","timestamp":1687308852000},"page":"1168-1183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Spray\u2010induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0641-0548","authenticated-orcid":false,"given":"Amanda G.","family":"McRae","sequence":"first","affiliation":[{"name":"Department of Plant and Microbial Biology University of California  Berkeley California 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