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Here we investigate the roles of the alternative iron acquisition system, reductive iron assimilation (RIA), via disruption of the\n                    <jats:italic>fetC<\/jats:italic>\n                    gene, which encodes a multicopper ferroxidase, either alone (i.e., \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    ) or in combination with disruption of the gene\n                    <jats:italic>sidA<\/jats:italic>\n                    , which encodes a siderophore biosynthesis enzyme (i.e., \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>sidA<\/jats:italic>\n                    ). The phenotypic characteristics of these mutants were compared to \u0394\n                    <jats:italic>sidA<\/jats:italic>\n                    and wild\u2010type (WT) strains during growth under axenic culture conditions (in culture) and in symbiosis with the host grass, perennial ryegrass (in planta). Under iron deficiency, the colony growth rate of \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    was slightly slower than that of WT, while the growth of \u0394\n                    <jats:italic>sidA<\/jats:italic>\n                    and \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>sidA<\/jats:italic>\n                    mutants was severely suppressed. Siderophore analyses indicated that \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    mutants hyperaccumulate ferriepichlo\u00ebnin A (FEA) at low iron concentrations and ferricrocin and FEA at higher iron concentrations. When compared to WT, all mutant strains displayed hyperbranching hyphal structures and a reduced ratio of\n                    <jats:italic>Epichlo\u00eb<\/jats:italic>\n                    DNA to total DNA in planta. Furthermore, host colonization and vertical transmission through infection of the host seed were significantly reduced in the \u0394\n                    <jats:italic>fetC<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>sidA<\/jats:italic>\n                    mutants, confirming that high\u2010affinity iron uptake is a critical process for\n                    <jats:italic>Epichlo\u00eb<\/jats:italic>\n                    transmission. Thus, RIA and siderophore iron uptake are complementary systems required for the maintenance of iron metabolism, fungal growth, and symbiosis between\n                    <jats:italic>E. festucae<\/jats:italic>\n                    and perennial ryegrass.\n                  <\/jats:p>","DOI":"10.1111\/mpp.13379","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T07:35:33Z","timestamp":1689924933000},"page":"1430-1442","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010affinity iron uptake is required for optimal\n                    <i>Epichlo\u00eb festucae<\/i>\n                    colonization of\n                    <i>Lolium perenne<\/i>\n                    and seed transmission"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2959-4482","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"AgResearch Limited, Grasslands Research Centre  Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha T.","family":"Forester","sequence":"additional","affiliation":[{"name":"AgResearch Limited, Grasslands Research Centre  Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma R.","family":"Applegate","sequence":"additional","affiliation":[{"name":"AgResearch Limited, Grasslands Research Centre  Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqi","family":"Liu","sequence":"additional","affiliation":[{"name":"AgResearch Limited, Grasslands Research Centre  Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"AgResearch Limited, Grasslands Research Centre  Palmerston North New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-01-13-0003-R"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12561"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00380768.2014.922406"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-14-0183-R"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2990"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jac.12269"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2134\/agronj1993.00021962008500010011x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02503-14"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof6040322"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S095375620100510X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7012(83)90033-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-11-13-0328-R"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf901028z"},{"key":"e_1_2_7_15_1","unstructured":"deMendiburu F.&deMendiburu M.F.(2019)Package \u2018agricolae.\u2019 R Package Version 1\u20132. 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