{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:46:41Z","timestamp":1792615601034,"version":"4.5.0"},"reference-count":78,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015401","name":"Key Research and Development Projects of Shaanxi Province","doi-asserted-by":"publisher","award":["2021LLRH\u201007"],"award-info":[{"award-number":["2021LLRH\u201007"]}],"id":[{"id":"10.13039\/501100015401","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Phytophthora infestans<\/jats:italic>\n                    is a notorious oomycete pathogen that causes potato late blight. It secretes numerous effector proteins to manipulate host immunity. Understanding mechanisms underlying their host cell manipulation is crucial for developing disease resistance strategies. Here, we report that the conserved RXLR effector Pi05910 of\n                    <jats:italic>P. infestans<\/jats:italic>\n                    is a genotype\u2010specific avirulence elicitor on potato variety Longshu 12 and contributes virulence by suppressing and destabilizing host glycolate oxidase StGOX4. By performing co\u2010immunoprecipitation, yeast\u2010two\u2010hybrid assays, luciferase complementation imaging, bimolecular fluorescence complementation and isothermal titration calorimetry assays, we identified and confirmed potato StGOX4 as a target of Pi05910. Further analysis revealed that\n                    <jats:italic>StGOX4<\/jats:italic>\n                    and its homologue\n                    <jats:italic>NbGOX4<\/jats:italic>\n                    are positive immune regulators against\n                    <jats:italic>P. infestans<\/jats:italic>\n                    , as indicated by infection assays on potato and\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    overexpressing\n                    <jats:italic>StGOX4<\/jats:italic>\n                    and TRV\u2010\n                    <jats:italic>NbGOX4<\/jats:italic>\n                    plants.\n                    <jats:italic>StGOX4<\/jats:italic>\n                    \u2010mediated disease resistance involves enhanced reactive oxygen species accumulation and activated the salicylic acid signalling pathway. Pi05910 binding inhibited enzymatic activity and destabilized StGOX4. Furthermore, mutagenesis analyses indicated that the 25th residue (tyrosine, Y25) of StGOX4 mediates Pi05910 binding and is required for its immune function. Our results revealed that the core RXLR effector of\n                    <jats:italic>P. infestans<\/jats:italic>\n                    Pi05910 suppresses plant immunity by targeting StGOX4, which results in decreased enzymatic activity and protein accumulation, leading to enhanced plant susceptibility.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70021","type":"journal-article","created":{"date-parts":[[2024,11,2]],"date-time":"2024-11-02T02:29:26Z","timestamp":1730514566000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The\n                    <i>Phytophthora infestans<\/i>\n                    effector Pi05910 suppresses and destabilizes host glycolate oxidase\n                    <scp>StGOX4<\/scp>\n                    to promote plant susceptibility"],"prefix":"10.1111","volume":"25","author":[{"given":"Peiling","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuhong","family":"Gou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"},{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwen","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assiya","family":"Ansabayeva","sequence":"additional","affiliation":[{"name":"Department of Agronomy A. Baitursynov Kostanay Regional University  Kostanay Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7286-4041","authenticated-orcid":false,"given":"Weixing","family":"Shan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12932"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009388"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.02.009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914408107"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020260"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-04-23-0051-R"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19902"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137071"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00692"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048616e"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15840"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-023-01391-z"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2020.01.004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1139\/gen-2023-0002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16861"},{"key":"e_1_2_7_17_1","first-page":"1975","article-title":"Phytophthora infestans RXLR effector AVR1 interacts with exocyst component Sec5 to manipulate plant immunity","volume":"169","author":"Du Y.","year":"2015","journal-title":"Plant Physiology"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00919"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17828"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2018.05.009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.043008.091948"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad005"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14472"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-07-22-0160-TA"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad449"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00028910"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15792"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adh5021"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022110682"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15700"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12760"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19194"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05286"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2023.108231"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2019.118609"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23084224"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13176"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.009"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2019.01.018"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13416"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12883"},{"key":"e_1_2_7_42_1","unstructured":"Li X.(2017)Regulation and function of association\u2010dissociation of glycolate oxidase with catalase in rice[PhD thesis]. 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Yangling: The Northwest A&F University."},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12915"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.20.00092"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2019.02.004"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.02.002"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34648-0"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.70021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:03:23Z","timestamp":1762272203000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.70021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":78,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/mpp.70021"],"URL":"https:\/\/doi.org\/10.1111\/mpp.70021","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"2024-05-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70021"}}