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In this study, we engineered four strains of\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)\u2010expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E\u2010flg22 strain expressed a secreted EGFP\u2010Flg22 fusion protein, the UW4E\u2010Flg(flg22) strain expressed a non\u2010secreted Flg22, and the UW4E\u2010flg22\u2010D strain expressed a secreted Flg22\u2010DNase fusion protein. UW4E\u2010flg22 and UW4E\u2010flg22\u2010D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22\u2010secreting strains showed a 33.8%\u201393.8% higher colonization of wheat root tips and reduced the root rot incidence caused by\n                    <jats:italic>Rhizoctonia solani<\/jats:italic>\n                    and\n                    <jats:italic>Fusarium pseudograminearum<\/jats:italic>\n                    by 24.6%\u201335.7% compared to the non\u2010Flg22\u2010secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70026","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:39:10Z","timestamp":1730767150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Flg22\u2010facilitated\n                    <scp>PGPR<\/scp>\n                    colonization in root tips and control of root rot"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9745-3350","authenticated-orcid":false,"given":"Yanan","family":"Li","sequence":"first","affiliation":[{"name":"College of Life Sciences Henan Agricultural University, Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs  Zhengzhou 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