{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T22:44:29Z","timestamp":1773269069357,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972930"],"award-info":[{"award-number":["31972930"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170073"],"award-info":[{"award-number":["32170073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32370076"],"award-info":[{"award-number":["32370076"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptomyces scabies<\/jats:italic>\n                    is a well\u2010researched plant pathogen belonging to the genus\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    . Its virulence is linked to the production of the secondary metabolite thaxtomin A, which is tightly regulated at the transcriptional level. The leucine\u2010responsive regulatory protein (Lrp) family is conserved in prokaryotes and is involved in various crucial biological processes. However, the regulatory interaction between Lrp protein and pathogenic\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    species remains poorly understood. This study aims to explore the role of SCAB_Lrp2 in regulating thaxtomin biosynthesis and pathogenicity, and to analyse the shared and unique features of Lrp homologues in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    . We observed that SCAB_Lrp2 (SCAB_75421) showed significant homology with SCAB_Lrp, a recognised activator of thaxtomin A production in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    . Our results revealed a regulatory interaction between SCAB_Lrp2 and SCAB_Lrp in terms of their targets, although SCAB_Lrp2 does not respond to the amino acid\u2010effectors of SCAB_Lrp. In contrast to SCAB_Lrp, deletion of\n                    <jats:italic>SCAB_Lrp2<\/jats:italic>\n                    resulted in a notable increase in thaxtomin A production with the emergence of a hypervirulent phenotype in\n                    <jats:italic>S. scabies<\/jats:italic>\n                    . Further analysis revealed that SCAB_Lrp2 represses the transcription of the thaxtomin biosynthetic gene cluster by directly regulating the cluster\u2010situated regulator (CSR) gene\n                    <jats:italic>txtR<\/jats:italic>\n                    . Moreover, the precursor of thaxtomin, tryptophan, acts as an effector of SCAB_Lrp2, strengthening the repressive effect on thaxtomin biosynthesis through\n                    <jats:italic>txtR<\/jats:italic>\n                    . These findings provide new insights into the functional conservation and diversity of Lrp homologues involved in the biosynthesis of thaxtomin phytotoxins in pathogenic\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    species.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70036","type":"journal-article","created":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T23:35:28Z","timestamp":1733096128000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in\n                    <i>Streptomyces scabies<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"Haoyang","family":"He","sequence":"first","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Institute of Physical Science and Information Technology Anhui University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingrui","family":"Song","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youquan","family":"Zou","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueyan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Endong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hang","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Institute of Physical Science and Information Technology Anhui University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buchang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Institute of Physical Science and Information Technology Anhui University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3213-2978","authenticated-orcid":false,"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern344"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02018-14"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-011-9943-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.249"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03504-15"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05942.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27144"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2009.04.013"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2009.00561.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000818"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-019-02178-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2016.10.012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8339-9"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-021-11228-8"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13285"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-008-9240-4"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.44.032905.091147"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2020.1807461"},{"key":"e_1_2_8_20_1","first-page":"417","article-title":"Increasing the Efficiency of Heterologous Promoters in Actinomycetes","volume":"4","author":"Wilkinson C. 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