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POZ\/BTB containing\u2010protein1 (POB1) is a type of BTB\u2010BACK domain\u2010containing E3 ligase, which was previously reported to be a negative regulator of defence responses in multiple plant species. In this report, we identified MdPOB1\u2010like (MdPOB1L) as a positive regulator in defence responses against\n                    <jats:italic>Botryosphaeria dothidea<\/jats:italic>\n                    by manipulating protein stability of MdNPR1, a master regulator in salicylic acid (SA) signalling pathway, in apple (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Malus domestica<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). We first found that MdPOB1L is evolutionarily close to PbPOB1 from\n                    <jats:italic>Pyrus bretschneideri<\/jats:italic>\n                    , and it was localised in the nucleus in the epidermal cells of\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    leaves. Reverse transcription\u2010quantitative PCR analysis revealed that\n                    <jats:italic>MdPOB1L<\/jats:italic>\n                    was inducible upon\n                    <jats:italic>B. dothidea<\/jats:italic>\n                    infection or SA treatment. Further investigation demonstrated that overexpressing\n                    <jats:italic>MdPOB1L<\/jats:italic>\n                    enhanced disease resistance to\n                    <jats:italic>B. dothidea<\/jats:italic>\n                    in both apple calli and fruits, while repressing its transcription displayed the opposite phenotype. Moreover, overexpressing\n                    <jats:italic>MdPOB1L<\/jats:italic>\n                    promoted the transcription of SA\u2010responsive pathogenesis\u2010related (\n                    <jats:italic>PR<\/jats:italic>\n                    ) genes and\n                    <jats:italic>MdNPR1<\/jats:italic>\n                    . We further demonstrated that MdPOB1L interacted with MdNPR1 in the nucleus and promoted its ubiquitination and degradation through the proteasome pathway, which might contribute to the turnover of MdNPR1 in the nucleus, leading to enhanced downstream responses of the SA signalling pathway. Therefore, we demonstrate here that MdPOB1L positively regulates defence responses against\n                    <jats:italic>B. dothidea<\/jats:italic>\n                    by manipulating MdNPR1 protein stability in apple.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70129","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T17:32:23Z","timestamp":1754587943000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>MdPOB1L<\/scp>\n                    Positively Regulates Disease Resistance Against\n                    <i>Botryosphaeria dothidea<\/i>\n                    by Manipulating\n                    <scp>MdNPR1<\/scp>\n                    Protein Stability in\n                    <i>Malus domestica<\/i>"],"prefix":"10.1111","volume":"26","author":[{"given":"Fujun","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"},{"name":"Department of Horticulture Key Laboratory of Special Fruits &amp;Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Group, College of Agriculture, Shihezi University  Shihezi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojian","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinsong","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Humic Acid Fertilizer, Ministry of Agriculture Shandong Agricultural University Fertilizer Science Tech. 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Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianzhen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Xuan","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangji","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient Production, National Key Laboratory of Wheat Improvement Shandong Agricultural University  Tai\u2010An Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Horticulture Hebei Agricultural University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxiang","family":"You","sequence":"additional","affiliation":[{"name":"College of Horticulture Science and Engineering, Apple Technology Innovation Center of Shandong Province, Shandong Collaborative Innovation Center of Fruit &amp; Vegetable Quality and Efficient 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