{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:12:50Z","timestamp":1788491570876,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470152"],"award-info":[{"award-number":["32470152"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1700701"],"award-info":[{"award-number":["2023YFD1700701"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["TD2022C003"],"award-info":[{"award-number":["TD2022C003"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Potato mop\u2010top virus (PMTV) is the causal agent of potato tuber spraing disease, which causes significant economic losses to potato production worldwide. The 3\u2032\u2010proximal end of PMTV genomic RNA3 encodes an 8\u2009kDa cysteine\u2010rich protein (8K) that is not essential for replication and movement but contributes to virus infection and symptom development. Here, we demonstrate that PMTV 8K forms endomembrane multimers, alters the membrane permeability of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and possesses potassium and proton conductance activity. In addition, our data reveal that two conserved cysteine residues in the central hydrophobic \u03b1\u2010helix are essential for the viroporin activity. These results not only deepen our understanding of the function of PMTV 8K but also provide new insights into the diversity and origin of plant viral viroporins.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70150","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T05:46:49Z","timestamp":1756878409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Small Cysteine\u2010Rich Protein of Potato Mop\u2010Top Virus Exhibits Viroporin Activity"],"prefix":"10.1111","volume":"26","author":[{"given":"Ye","family":"Liu","sequence":"first","affiliation":[{"name":"Potato Research Institute Heilongjiang Academy of Agricultural Sciences  Harbin Heilongjiang China"},{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yameng","family":"Luan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqin","family":"Ji","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naihui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9771-9059","authenticated-orcid":false,"given":"Xiaofei","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanju","family":"Bai","sequence":"additional","affiliation":[{"name":"Potato Research Institute Heilongjiang Academy of Agricultural Sciences  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2014.07.030"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2004.00246.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2401748121"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-12-0142-R"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.17317"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.6.11.1593"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","unstructured":"Evans R. M.O'Neill A.Pritzel et\u00a0al.2022.\u201cProtein Complex Prediction With AlphaFold\u2010Multimer.\u201dbioRxiv.https:\/\/doi.org\/10.1101\/2021.10.04.463034. 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