{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T22:37:14Z","timestamp":1784327834819,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB\u20101906060"],"award-info":[{"award-number":["MCB\u20101906060"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MCB\u20102350392"],"award-info":[{"award-number":["MCB\u20102350392"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20102410009"],"award-info":[{"award-number":["IOS\u20102410009"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","award":["P4\u20100077"],"award-info":[{"award-number":["P4\u20100077"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007698","name":"University of Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007698","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    RNA silencing is one of the major defence activities against viral pathogens in plants. Silencing signals are initiated by Dicer\u2010like proteins (DCLs) to generate viral\u2010derived small RNAs (sRNAs). Viral sRNAs are then loaded into Argonaute proteins to form an RNA\u2010induced silencing complex to guide cleavage of target RNAs based on sequence homology. While the model regarding RNA silencing\u2010mediated defence against viral pathogens is largely established based on extensive studies using the model plant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:styled-content>\n                    , there are diverse sets of silencing components in other plants, especially in domesticated crops. Here, we tracked the expansion of solanaceous\u2010specific DCL2 genes during the course of evolution. We found that the\n                    <jats:italic>DCL2a<\/jats:italic>\n                    gene in tomato chromosome 6 is likely an evolutionarily new gene copy. We also found that\n                    <jats:italic>DCL2b<\/jats:italic>\n                    is more prone to be induced by viral pathogens in tomato plants, which is dependent on the combinations of cultivar and viral pathogen. Both DCL2a and DCL2b are critical to suppress the accumulation titre of a subviral agent, potato spindle tuber viroid (PSTVd). We noticed an unusually high accumulation of viral sRNAs shorter than 20\u2009nt (16\u2010 to 19\u2010nt in length) in viroid\u2010infected tomato cv. Heinz 1706. Using synthetic small interfering RNAs, we demonstrated that shorter size sRNAs may also play a role in suppressing target RNAs, which can be interfered with by a viral suppressor of silencing, P19. Altogether, we provided further insights into the expansion of functional DCL2 family members in the\n                    <jats:italic>Solanaceae<\/jats:italic>\n                    family and their roles in combating viral and subviral agents.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70173","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T04:23:41Z","timestamp":1762835021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evolutionarily New Solanaceous\n                    <scp>DCL<\/scp>\n                    2 Family Members Acquire Functions in Tomato"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3281-727X","authenticated-orcid":false,"given":"Jie","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Plant Pathology University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3733-8983","authenticated-orcid":false,"given":"Junfei","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4797-8105","authenticated-orcid":false,"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Food Science and Human Nutrition Department University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8907-1627","authenticated-orcid":false,"given":"Jernej","family":"Jak\u0161e","sequence":"additional","affiliation":[{"name":"Agronomy Department, Biotechnical Faculty University of Ljubljana  Ljubljana Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9659-977X","authenticated-orcid":false,"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Florida  Gainesville Florida USA"},{"name":"Plant Molecular and Cellular Biology Program University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050312-120043"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1011786"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-4811-8-41"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/mp\/sss118"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-10-22-0358-IA"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-014-2158-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0071-x"},{"key":"e_1_2_6_9_1","article-title":"Transcriptome Analysis Reveals Dynamic Cultivar\u2010Dependent Patterns of Gene Expression in Potato Spindle Tuber Viroid\u2010Infected Pepper","volume":"10","author":"Hadjieva 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