{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:00:03Z","timestamp":1789610403846,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T00:00:00Z","timestamp":1765152000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recent studies proposed that the negative strands of some single\u2010stranded, positive\u2010sense RNA viruses contain reverse open reading frames (rORFs) that encode functional peptides. Highly conserved rORFs were found in the negative strand of viruses within the\n                    <jats:italic>Potyviridae<\/jats:italic>\n                    family, including turnip mosaic virus (TuMV), where rORF2 was shown to be essential for infection and survival. In this study, we analysed the existence and potential functionality of rORFs in plum pox virus (PPV) using mass spectrometry and mutagenesis. Unbiased mass spectrometry analyses failed to identify any rORF\u2010encoded peptides and PPV mutants abolishing the expression of individual rORF\u2010derived peptides showed no significant difference in infection rates compared to the wild\u2010type virus in the experimental host\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    or in the natural host\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Prunus persica<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Furthermore, competitive infection experiments revealed that neither the wild type nor the rORF2 knockout mutant, which had proven lethal when tested in TuMV, exhibited any competitive advantage over the other. Taken together, these findings suggest that rORFs in PPV are either not produced or are functionally irrelevant for infection, and they highlight the need to evaluate the role of rORFs in other potyviruses on a case\u2010by\u2010case basis.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70179","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T07:16:25Z","timestamp":1765264585000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating the Negative\u2010Strand Coding Potential in Plum Pox Virus"],"prefix":"10.1111","volume":"26","author":[{"given":"Beatriz","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Vicente","family":"Del \u00c1lamo","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Rinc\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Gonzalo","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9771-9059","authenticated-orcid":false,"given":"Xiaofei","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northeast Agricultural University  Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Antonio","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Sim\u00f3n\u2010Mateo","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3266-7200","authenticated-orcid":false,"given":"Adri\u00e1n A.","family":"Valli","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6541-2589","authenticated-orcid":false,"given":"Bernardo","family":"Rodamilans","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica Molecular de Plantas, Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC) Campus Universidad Aut\u00f3noma de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-08-11-0216"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800468105"},{"key":"e_1_2_5_4_1","article-title":"A Case for a Negative\u2010Strand Coding Sequence in a Group of Positive\u2010Sense RNA Viruses","volume":"6","author":"Dinan A. 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