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In this study, we have identified the key regulatory module, FaMYB63\/FvWRKY75\u2010\n                    <jats:italic>PR10.14<\/jats:italic>\n                    , that confers enhanced powdery mildew resistance in transgenic strawberry (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Fragaria vesca<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). FaMYB63, an R2R3\u2010MYB transcription factor, was induced by\n                    <jats:italic>P. aphanis<\/jats:italic>\n                    infection and responsive to the application of defence signalling molecules, including salicylic acid (SA), methyl jasmonate (JA), abscisic acid (ABA), and 1\u2010aminocyclopropane\u20101\u2010carboxylic acid (ACC). Silencing of\n                    <jats:italic>FaMYB63<\/jats:italic>\n                    led to reduced SA levels and increased powdery mildew susceptibility, accompanied by suppressed reactive oxygen species (ROS) bursts and down\u2010regulation of\n                    <jats:italic>PR10.14<\/jats:italic>\n                    expression. Conversely, overexpressing\n                    <jats:italic>PR10.14<\/jats:italic>\n                    in transgenic lines inhibited\n                    <jats:italic>P. aphanis<\/jats:italic>\n                    spore germination and enhanced ROS accumulation, indicating a dual role in direct pathogen inhibition and hypersensitive response\u2010triggered defence. Yeast one\u2010hybrid, electrophoretic mobility shift assay, \u03b2\u2010glucuronidase, and luciferase assays confirmed that FaMYB63 and FvWRKY75 were directly bound to the MYB\u2010binding sites and W\u2010box of the\n                    <jats:italic>PR10.14<\/jats:italic>\n                    promoter, respectively, and activated its transcription, while WRKY75 negatively regulated the expression of\n                    <jats:italic>MYB63<\/jats:italic>\n                    .\n                    <jats:italic>PR10.14<\/jats:italic>\n                    exhibited tissue\u2010specific expression, with the highest levels in red\u2010ripening fruits, suggesting a role in developmental\u2010stage\u2010dependent defence. These findings suggest FaMYB63 as an SA\u2010dependent regulator of\n                    <jats:italic>PR10.14<\/jats:italic>\n                    \u2010mediated resistance, bridging hormone signalling and pathogen response. This study provides a molecular target for breeding powdery mildew\u2010resistant strawberry cultivars through genetic engineering approaches, offering an alternative to fungicides for sustainable and environmental disease management in horticultural crops and advances our understanding of MYB\u2010 or WRKY\u2010PR10 networks in plant immunity.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70186","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T07:27:02Z","timestamp":1765265222000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>FaMYB63<\/scp>\n                    and\n                    <scp>FvWYRKY75<\/scp>\n                    Activate\n                    <i>FvPR10.14<\/i>\n                    Boosting Strawberry Immunity Against Powdery Mildew"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0992-9479","authenticated-orcid":false,"given":"Rongyi","family":"Jiang","sequence":"first","affiliation":[{"name":"School of Horticulture Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Tao","sequence":"additional","affiliation":[{"name":"School of Horticulture Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingbin","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Horticulture Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Horticulture Anhui Agricultural University  Hefei 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