{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:59:08Z","timestamp":1789095548882,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T00:00:00Z","timestamp":1768953600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CaM\u2010binding Protein 60\u2010like G (CBP60g) and Systemic Acquired Resistance Deficient 1 (SARD1) are key immune signalling regulators that redundantly promote salicylic acid (SA) biosynthesis and plant immunity. Pathogen effectors often target these immune nodes to suppress plant defence. However, the role of bacterial effectors in disabling CBP60g and SARD1 to increase plant susceptibility remains unclear. In this study, we show that RipAW, an E3 ligase effector from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ralstonia solanacearum<\/jats:italic>\n                    <\/jats:styled-content>\n                    , induces root architecture changes and enhances plant susceptibility to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R.\u2009solanacearum<\/jats:italic>\n                    <\/jats:styled-content>\n                    in\n                    <jats:italic>Est::RipAW<\/jats:italic>\n                    transgenic plants. The constitutively expressed\n                    <jats:italic>RipAW (C177S)<\/jats:italic>\n                    , lacking E3 ligase activity, did not affect root architecture or plant susceptibility, indicating that RipAW's E3 ligase activity is crucial for these phenotypes. Transcriptional profiling of\n                    <jats:italic>Est::RipAW<\/jats:italic>\n                    plants revealed strong up\u2010regulation of\n                    <jats:italic>CBP60g<\/jats:italic>\n                    and\n                    <jats:italic>SARD1,<\/jats:italic>\n                    while the SA signalling pathway remained in a basal state. Transient expression of\n                    <jats:italic>RipAW<\/jats:italic>\n                    and\n                    <jats:italic>CBP60g<\/jats:italic>\n                    in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    showed that RipAW associates with CBP60g and affects its stability. Genetic analysis revealed that loss\u2010of\u2010function mutations in\n                    <jats:italic>CBP60g<\/jats:italic>\n                    and\n                    <jats:italic>SARD1<\/jats:italic>\n                    increased plant susceptibility to\n                    <jats:italic>R.\u2009solanacearum,<\/jats:italic>\n                    but did not enhance RipAW\u2010mediated pathogen growth. Furthermore, growth of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R.\u2009solanacearum<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u0394\n                    <jats:italic>RipAW<\/jats:italic>\n                    null mutant strain was reduced in wild\u2010type plants but restored in\n                    <jats:italic>cbp60g\/sard1<\/jats:italic>\n                    mutant plants, confirming that the promotion of RipAW on bacterial growth is dependent on CBP60g and SARD1. Surprisingly, CBP60g and SARD1 were not involved in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R.\u2009solanacearum<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010induced and RipAW\u2010triggered root architecture changes. Overall, our findings demonstrate that RipAW increases plant susceptibility to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>R.\u2009solanacearum<\/jats:italic>\n                    <\/jats:styled-content>\n                    via both CBP60g\/SARD1\u2010dependent and \u2010independent pathways.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70207","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:06:23Z","timestamp":1769061983000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Effector\n                    <scp>RipAW<\/scp>\n                    Enhances\n                    <i>Ralstonia solanacearum<\/i>\n                    Invasion in\n                    <i>Arabidopsis<\/i>\n                    via\n                    <scp>CBP60g<\/scp>\n                    \/\n                    <scp>SARD1<\/scp>\n                    \u2010Dependent and \u2010Independent Pathways"],"prefix":"10.1111","volume":"27","author":[{"given":"Huijuan","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Agronomy Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouyang","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Agronomy Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production, College of Agronomy Northwest A&amp;F University  Yangling 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