{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:04:36Z","timestamp":1779901476986,"version":"3.53.1"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006031","name":"United States - Israel Binational Agricultural Research and Development Fund","doi-asserted-by":"publisher","award":["5444\u201021"],"award-info":[{"award-number":["5444\u201021"]}],"id":[{"id":"10.13039\/100006031","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Postharvest fungal diseases cause substantial losses in horticultural crops and are commonly managed using chemical fungicides, which raise concerns regarding resistance development and environmental impact. Plant\u2010derived antifungal peptides represent a promising alternative for sustainable disease control. Here, we evaluated the antifungal activity and mode of action of three nodule\u2010specific cysteine\u2010rich (NCR) peptides\u2014NCR044, NCR192 and NCR13_PFV2\u2014against major postharvest fungal pathogens. Among the peptides tested, NCR13_PFV2 exhibited the strongest inhibitory activity against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Alternaria alternata<\/jats:italic>\n                    <\/jats:styled-content>\n                    ,\n                    <jats:italic>Colletotrichum gloeosporioides<\/jats:italic>\n                    ,\n                    <jats:italic>Lasiodiplodia theobromae<\/jats:italic>\n                    and\n                    <jats:italic>Penicillium expansum<\/jats:italic>\n                    , although activity against\n                    <jats:italic>C. gloeosporioides<\/jats:italic>\n                    was predominantly fungistatic rather than fungicidal.\u00a0Mechanistic analyses using\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. alternata<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a model pathogen revealed that NCR13_PFV2 rapidly associates with fungal membranes, induces membrane permeabilization and exhibits selective binding to anionic phospholipids and phosphoinositides. Peptide treatment was associated with mitochondrial depolarization, elevated reactive oxygen species accumulation, reduced cellular respiration and disruption of vacuolar integrity. These intracellular effects were accompanied by loss of turgor pressure and hyphal collapse. Importantly, application of NCR13_PFV2 to wounded bell pepper fruit significantly reduced lesion development caused by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. alternata<\/jats:italic>\n                    <\/jats:styled-content>\n                    in a concentration\u2010dependent manner. Together, these findings demonstrate that NCR13_PFV2 suppresses postharvest fungal infection through a multi\u2010target mechanism that disrupts fungal cellular homeostasis, highlighting its potential as a peptide\u2010based alternative to synthetic fungicides.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70266","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T08:34:26Z","timestamp":1779093266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Chickpea\n                    <scp>NCR<\/scp>\n                    Peptide Suppresses Postharvest\n                    <i>Alternaria alternata<\/i>\n                    Infection by Disrupting Fungal Membranes, Mitochondria and Respiration"],"prefix":"10.1111","volume":"27","author":[{"given":"Alon","family":"Shomron","sequence":"first","affiliation":[{"name":"Department of Postharvest Science ARO, Volcani Center  Rishon LeZion Israel"},{"name":"Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0110-2888","authenticated-orcid":false,"given":"Raviraj","family":"Kalunke","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Godwin","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilip","family":"Shah","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noam","family":"Alkan","sequence":"additional","affiliation":[{"name":"Department of Postharvest Science ARO, Volcani Center  Rishon LeZion Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14025"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12460"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001981"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0181"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13708"},{"key":"e_1_2_10_7_1","first-page":"179","volume-title":"The Citrus Industry","author":"Eckert J. 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