{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:02:32Z","timestamp":1785214952156,"version":"3.55.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470149"],"award-info":[{"award-number":["32470149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272483"],"award-info":[{"award-number":["32272483"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The 68th amino acid residue (cysteine, C68) in tobacco mosaic virus (TMV) movement protein (MP) and the corresponding position in some tobamoviruses are required to trigger\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \u2010mediated resistance. In contrast, the 67th amino acid residue (histidine, H67) in tomato brown rugose fruit virus (ToBRFV) MP enables evasion, although its key property remains unclear. To assess the key property of this site, a saturated mutant pool was generated at ToBRFV MP H67 and TMV MP C68, along with two single mutants, H67C and C68H. Infection assays were conducted using two tomato cultivars, Jingpeng No. 1 (JP,\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \/\n                    <jats:italic>tm\u20102<\/jats:italic>\n                    ) and Rutgers (RG,\n                    <jats:italic>tm\u20102<\/jats:italic>\n                    \/\n                    <jats:italic>tm\u20102<\/jats:italic>\n                    ), as well as wild\u2010type (WT) and\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    transgenic\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    . Progeny sequencing revealed species\u2010 and cultivar\u2010specific outcomes. The mutants TAC and TAT (encoding the aromatic amino acid tyrosine, Y) and CAC (encoding\u00a0the WT histidine, H), were the only ones detected in JP tomato and\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \u2009\n                    <jats:italic>N. benthamiana<\/jats:italic>\n                    . In single\u2010infection assays, ToBRFV carrying TTC (encoding phenylalanine, F) at this position infected JP tomato, TAC (Y) enabled infection of\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \u2009\n                    <jats:italic>N. benthamiana.<\/jats:italic>\n                    TGG (encoding tryptophan, W) infected both JP tomato and\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \u2009\n                    <jats:italic>N. benthamiana.<\/jats:italic>\n                    Moreover, CAT (H) but not CAC (H), and TTC (F) but not TTT (F) infected JP plants, highlighting the role of the critical RNA motif. This suggests that the aromatic moiety and critical RNA motif at this position are essential for evading\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    \u2010mediated resistance, as the imidazole ring of the WT histidine also exhibits aromatic character. Therefore,\n                    <jats:italic>Tm\u20102<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sup>\n                    may be engineered to recognize aromatic amino acids at this position to confer resistance to ToBRFV.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70290","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T04:40:04Z","timestamp":1783917604000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Both Conserved Aromatic Nature and Critical\n                    <scp>RNA<\/scp>\n                    Motif of Residue 67 in the\n                    <scp>ToBRFV<\/scp>\n                    Movement Protein May Be Essential for Evading\n                    <i>\n                      Tm\u20102\n                      <sup>2<\/sup>\n                    <\/i>\n                    \u2010Mediated Resistance"],"prefix":"10.1111","volume":"27","author":[{"given":"Yuhong","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing China"},{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchen","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Nie","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luyou","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0049-8588","authenticated-orcid":false,"given":"Fei","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3849-4080","authenticated-orcid":false,"given":"Jianping","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing China"},{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6241-8159","authenticated-orcid":false,"given":"Jian","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40858-021-00439-z"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.4c00606"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-72298-y"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-022-02629-0"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13318"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-01-21-0023-R"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13385"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-022-01921-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-016-0676-2"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170429"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-018-3819-5"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00411"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.984846"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42161-022-01287-9"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr60290a003"},{"key":"e_1_2_10_17_1","unstructured":"Tanwir F.2012.Analysis of the Structure of Tomato Mosaic Virus Movement Protein Based on Virus Host Interactions. [PhD Thesis]. University of Hohenheim."},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-05-22-0167-RVW"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.002176"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-025-3026-7"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.1998.11.6.498"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.11.6432-6438.1993"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koae012"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2024.110137"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012958"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008147"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012142"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa100"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13115"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12040857"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70559"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1382862"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.70290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mpp.70290","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.70290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:56:01Z","timestamp":1785214561000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.70290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":31,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/mpp.70290"],"URL":"https:\/\/doi.org\/10.1111\/mpp.70290","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70290"}}