{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:02:32Z","timestamp":1785214952057,"version":"3.55.0"},"reference-count":82,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mutants in the eukaryotic initiation factor 4E (\n                    <jats:italic>eIF4E<\/jats:italic>\n                    ) gene family provide recessive resistance to plant viruses. Previously, we demonstrated that knocking out\n                    <jats:italic>SteIF4E1<\/jats:italic>\n                    potato alleles (\n                    <jats:italic>A, B, B, B1<\/jats:italic>\n                    ) resulted in limited resistance to a necrogenic PVY isolate (NTN). Expanding on this, we used a non\u2010transgenic method employing Cas12a and a single\u2010stranded oligodeoxynucleotide (ssODN) as a repair template to introduce amino acid changes in SteIF4E1, attempting to mimic the\n                    <jats:italic>Capsicum<\/jats:italic>\n                    PVY resistance allele\n                    <jats:italic>pvr2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>1<\/jats:italic>\n                    <\/jats:sup>\n                    . However, no plants that had been regenerated from transfected protoplast cells displayed correct\n                    <jats:italic>SteIF4E1<\/jats:italic>\n                    editing. Instead, truncated sequence duplications (pvr2\n                    <jats:sup>1<\/jats:sup>\n                    SD) appeared at some mutated alleles, suggesting that ssODN\u2010mediated repair predominantly occurs via microhomology\u2010mediated end joining. Importantly, out of the 39 edited lines tested, the dwarf line Bb29 exhibited resistance to PVY\u2010NTN and showed reduced systemic infection sustained by PVY\u2010O. Notably, PVY\u2010NTN resistance\u2010breaking isolates occasionally emerged in Bb29, confirming genuine eIF4E\u2010mediated resistance. In Bb29, three of four\n                    <jats:italic>eIF4E1<\/jats:italic>\n                    copies were mutated (\n                    <jats:italic>A\u039412, B\u03946, B, B1pvr2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>1<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>SD<\/jats:italic>\n                    ), with\n                    <jats:italic>SteIF4E1_B1pvr2<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>1<\/jats:italic>\n                    <\/jats:sup>\n                    <jats:italic>SD<\/jats:italic>\n                    featuring a premature stop codon at the 5\u2032 end of the mutated sequence, and the unique\n                    <jats:italic>SteIF4E1_A\u039412<\/jats:italic>\n                    allele in our gene\u2010edited lines. We found that\n                    <jats:italic>SteIF4E1_B\u03946<\/jats:italic>\n                    , but not\n                    <jats:italic>SteIF4E1_A\u039412<\/jats:italic>\n                    , partially complemented an eIF4E\u2010deficient yeast strain. In addition, introducing a wild\u2010type copy of\n                    <jats:italic>SteIF4E1<\/jats:italic>\n                    into Bb29 restored virus susceptibility and partially reversed dwarfism. Conversely, transforming wild\u2010type potatoes with\n                    <jats:italic>SteIF4E1_A\u039412<\/jats:italic>\n                    mimicked the resistance profile of Bb29, conferring stronger and broader resistance against PVY than the\n                    <jats:italic>SteIF4E1<\/jats:italic>\n                    knockout approach. These findings reveal a dominant\u2010negative resistance mechanism, opening new avenues for engineering potyvirus resistance.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70305","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:23:26Z","timestamp":1782804206000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Imprecise Cas12a\/\n                    <scp>ssODN<\/scp>\n                    \u2010Mediated Editing of\n                    <i>eIF4E1<\/i>\n                    Confers Dominant\u2010Negative Resistance to Potato Virus Y in\n                    <i>Solanum tuberosum<\/i>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1012-5547","authenticated-orcid":false,"given":"Alessandra","family":"Lucioli","sequence":"first","affiliation":[{"name":"Division Biotechnologies, ENEA\u2014Italian National Agency for New Technologies Energy and Sustainable Economic Development, Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9064-0495","authenticated-orcid":false,"given":"Raffaela","family":"Tavazza","sequence":"additional","affiliation":[{"name":"Division Biotechnologies, ENEA\u2014Italian National Agency for New Technologies Energy and Sustainable Economic Development, Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1044-6327","authenticated-orcid":false,"given":"Attila","family":"Molnar","sequence":"additional","affiliation":[{"name":"Institute of Molecular Plant Sciences University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3657-1158","authenticated-orcid":false,"given":"Vincenza","family":"Ilardi","sequence":"additional","affiliation":[{"name":"Research Centre for Plant Protection and Certification (CREA\u2010DC) Council for Agricultural Research and Economics (CREA)  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8675-5065","authenticated-orcid":false,"given":"Ad\u00e9la","family":"P\u0159ibylov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Experimental Plant Biology, Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-1064","authenticated-orcid":false,"given":"Mario","family":"Tavazza","sequence":"additional","affiliation":[{"name":"Division Biotechnologies, ENEA\u2014Italian National Agency for New Technologies Energy and Sustainable Economic Development, Casaccia Research Center  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","unstructured":"Agatep R. 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