{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:02:56Z","timestamp":1785214976410,"version":"3.55.0"},"reference-count":10,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Receptor\u2010like protein AtRLP23 from\n \n Arabidopsis thaliana<\/jats:italic>\n <\/jats:styled-content>\n perceives the conserved microbial peptide nlp24 derived from necrosis\u2010 and ethylene\u2010inducing peptide 1\u2010like proteins (NLPs), but immune outputs are often weak in heterologous systems. Here, we engineered RLP23\/Cf\u20109 chimeric receptors by fusing the extracellular domain of AtRLP23 to either the Cf\u20109 intracellular region (IC) or the Cf\u20109 transmembrane\u2013intracellular region (TM\u2013IC), and assessed their activity in\n Nicotiana benthamiana<\/jats:italic>\n . In transient expression assays, AtRLP23 alone did not trigger hypersensitive cell death after infiltration with 10\u2009\u03bcM nlp24, whereas both chimeras conferred nlp24\u2010induced cell death, with RLP23\/Cf\u20109\n TM\u2010IC<\/jats:sup>\n producing markedly stronger macroscopic symptoms than RLP23\/Cf\u20109\n IC<\/jats:sup>\n . Stable transgenic\n N. benthamiana<\/jats:italic>\n expressing an untagged RLP23\/Cf\u20109\n TM\u2010IC<\/jats:sup>\n chimera showed enhanced nlp24 responsiveness, including stronger seedling growth inhibition and cell death after repeated 10\u2009\u03bcM nlp24 treatment. In these plants, virus\u2010induced gene silencing showed that chimera\u2010mediated cell death requires SOBIR1 and the helper NLR NRC3, and transient\n NRC3<\/jats:italic>\n expression further sensitized the response, enabling cell death after a single 0.1\u2009\u03bcM nlp24 treatment. In addition, the chimera significantly enhanced resistance to the oomycete\n Phytophthora nicotianae<\/jats:italic>\n . Together, these results identify RLP23\/Cf\u20109\n TM\u2010IC<\/jats:sup>\n as an effective chimeric receptor and reveal transient\n NRC3<\/jats:italic>\n expression as a practical step to further potentiate nlp24\u2010triggered output in\n N. benthamiana<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/mpp.70307","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:24:35Z","timestamp":1782804275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An RLP23\/Cf\u20109\n TM\u2010IC<\/sup>\n Chimeric Receptor Enhances nlp24\u2010Triggered Immunity and Resistance to\n Phytophthora nicotianae<\/i>\n in\n Nicotiana benthamiana<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"Xiaodie","family":"Huo","sequence":"first","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siying","family":"Pan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongju","family":"Xiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichi","family":"Zhong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Co\u2010Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.140"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010414"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2013.10.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.03.028"},{"key":"e_1_2_7_6_1","first-page":"14","article-title":"Phytophthora nicotianae (Syn. \n P. parasitica\n ): A Broad\u2010Host\u2010Range Oomycete With a Complex Pathogenic Lifestyle","volume":"17","author":"Panabi\u00e8res F.","year":"2016","journal-title":"Molecular Plant 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