{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:35:03Z","timestamp":1791549303218,"version":"4.3.4"},"reference-count":72,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100015776","name":"African Union Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015776","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rice is a staple crop primarily recognised for its high content of carbohydrates and proteins. Rice yellow mottle disease (RYMD) is a destructive disease affecting rice fields in sub\u2010Saharan Africa and is caused by the rice yellow mottle virus (RYMV). Development of virus\u2010resistant genotypes is a highly recommended and effective approach to controlling RYMV. A genetic approach that exploits recessive mutations in susceptibility (\n                    <jats:italic>S<\/jats:italic>\n                    ) genes may enhance resistance to the virus. Reports indicate that most rice genotypes grown in Kenya are vulnerable to RYMV infection. Genome editing has shown promise in enhancing agronomic traits in crops. We obtained enhanced resistance to RYMV in the\n                    <jats:italic>Indica<\/jats:italic>\n                    rice cv. IR2793\u201080\u201001 using the CRISPR\u2010Cas9 system. The\n                    <jats:italic>eIF(iso)4G<\/jats:italic>\n                    susceptibility gene was targeted because natural mutations in this gene confer recessive resistance to RYMV. A Cas9\u2010OseIF(iso)4G\u2010gRNA\u2010expressing vector targeting the\n                    <jats:italic>eIF(iso)4G<\/jats:italic>\n                    gene was introduced into rice calli via\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    \u2010mediated transformation. Ten T\n                    <jats:sub>2<\/jats:sub>\n                    homozygous mutant plant lines were assessed for their reaction to RYMV, and infection was significantly reduced. There were no significant differences in the agronomic characteristics between the T\n                    <jats:sub>2<\/jats:sub>\n                    mutant lines and the wild\u2010type plants. CRISPR\/Cas9\u2010mediated knockout alleles of the\n                    <jats:italic>eIF(iso)4G<\/jats:italic>\n                    gene conferred enhanced resistance to RYMV, which may be classified as partial. Findings underscore the need to embrace precise editing strategies, such as prime editing, to generate superior resistance alleles. Overall, the study provides an alternative resistance enhancement strategy that can create knockout resistance alleles that can be incorporated into breeding programmes for RYMV resistance.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70312","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:14:57Z","timestamp":1784182497000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Enhanced Rice Yellow Mottle Virus Resistance via\n                    <scp>CRISPR<\/scp>\n                    \/Cas9\u2010Targeted Mutagenesis of the Rice\n                    <i>eIF(iso)4G<\/i>\n                    Gene"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1826-7777","authenticated-orcid":false,"given":"Antony","family":"Kigaru","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Biotechnology Pan African University Institute for Basic Sciences, Technology and Innovation  Nairobi Kenya"},{"name":"Department of Crop Sciences Bukura Agricultural College  Kakamega Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elijah M.","family":"Ateka","sequence":"additional","affiliation":[{"name":"Department of Horticulture and Food Security Jomo Kenyatta University of Agriculture and Technology  Nairobi Kenya"},{"name":"Department of Plant, Animal and Food Sciences Jaramogi Odinga Oginga University of Science and Technology  Bondo Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanu R.","family":"Pappu","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology Washington State University  Pullman Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everlyne M.","family":"Nganga","sequence":"additional","affiliation":[{"name":"International Rice Research Institute  Nairobi 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Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","first-page":"71","article-title":"Hygromycin as Selective Marker in Agrobacterium\u2010Mediated Genetic Transformation of Indica Rice MR210","volume":"41","author":"Ahmad A.","year":"2013","journal-title":"Journal of Tropical Agriculture and Food 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