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In this study, we characterised the\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 protein (BASIDIN1 from\n                    <jats:italic>Moniliophthora roreri<\/jats:italic>\n                    ), a homologue of the BASIDIN effector protein from\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">M. perniciosa<\/jats:styled-content>\n                      .\n                    <\/jats:italic>\n                    Recombinant\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 (r\n                    <jats:italic>Mr<\/jats:italic>\n                    B1) induced morphological changes typical of necrosis in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    cell suspensions, including cell swelling, reduced GFP (green fluorescent protein) fluorescence, plasma membrane damage, and electrolyte leakage. Proteomic analysis of the cells indicated that r\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 treatment modulated proteins associated with cellular homeostasis, including reduced abundance of proteins related to energy metabolism and protein folding, such as BiP (Binding Immunoglobulin Protein), whose non\u2010detection was validated by western blotting. In cacao leaves, spraying with r\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 (12.5\u2009\u03bcM) resulted in necrotic lesions affecting 54% of the leaf area after 10\u2009days, increasing to 77% in the presence of 0.5% urea. At a lower concentration (5\u2009\u03bcM), the recombinant protein promoted hydrogen peroxide accumulation, supporting the induction of oxidative stress. The phenotypic response to r\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 varied among cacao genotypes with different levels of resistance to FPR and witches' broom disease, with the susceptible Catongo genotype being the most affected, while resistant genotypes, such as ICS\u201095 and Scavina\u20106, did not show visible symptoms. These results position\n                    <jats:italic>Mr<\/jats:italic>\n                    B1 among the few functionally characterised effector proteins in the\n                    <jats:italic>\n                      M. roreri\u2013\n                      <jats:styled-content style=\"fixed-case\">Theobroma cacao<\/jats:styled-content>\n                    <\/jats:italic>\n                    pathosystem and suggest that this effector may be further evaluated as a candidate marker of susceptibility in cacao breeding programmes.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70316","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T04:29:56Z","timestamp":1783916996000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The\n                    <i>Moniliophthora roreri<\/i>\n                    Effector\n                    <i>Mr<\/i>\n                    <scp>B1<\/scp>\n                    Affects Cellular Homeostasis and Induces Genotype\u2010Dependent Necrosis in\n                    <i>Theobroma cacao<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"Ta\u00eds Ara\u00fajo","family":"Santos","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lu\u00edza","family":"do Carmo Santos","sequence":"additional","affiliation":[{"name":"Embrapa Mandioca e Fruticultura (CNPMF)  Cruz das Almas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monaliza Mac\u00eado","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keilane Silva","family":"Farias","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Henrique","family":"de Carvalho Neto","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronan Xavier","family":"Corr\u00eaa","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uilson Vanderlei","family":"Lopes","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas do Cacau (CEPEC) Comiss\u00e3o Executiva do Plano da Lavoura Cacaueira (CEPLAC)  Itabuna Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Priminho","family":"Pirovani","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Biol\u00f3gicas, Centro de Biotecnologia e Gen\u00e9tica (CBG) Universidade Estadual de Santa Cruz (UESC)  Ilh\u00e9us Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants14040503"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12648"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12134"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-4875-7"},{"key":"e_1_2_10_6_1","volume-title":"Catalogue of Cacaos Conserved at CRC Genebank","author":"Bekele F. 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