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During infection,\n \n P.\u2009triticina<\/jats:italic>\n <\/jats:styled-content>\n secretes effectors proteins to manipulate host immunity. Here we identified and characterized the\n \n P.\u2009triticina<\/jats:italic>\n <\/jats:styled-content>\n \u00a0effector Pt3863, which is highly expressed during the early phase of infection and significantly enhances fungal virulence. We also identified the wheat receptor\u2010like cytoplasmic kinase TaRLCK176 as a target of Pt3863. Functional assays demonstrated that TaRLCK176 positively regulates wheat resistance against leaf rust and is required for chitin\u2010induced reactive oxygen species (ROS) accumulation. Pt3863 subverts this defence through a dual inhibitory mechanism: first, it suppresses TaRLCK176 phosphorylation; second, it promotes TaRLCK176 degradation via the ubiquitin\u201026S proteasome pathway. Host\u2010induced gene silencing\n Pt3863<\/jats:italic>\n attenuated\n \n P.\u2009triticina<\/jats:italic>\n <\/jats:styled-content>\n virulence, while its overexpression in transgenic wheat lines increased susceptibility to\n \n P.\u2009tritici<\/jats:italic>\n <\/jats:styled-content>\n . Conversely, virus\u2010induced gene silencing of\n TaRLCK176<\/jats:italic>\n compromised wheat resistance. Our findings establish TaRLCK176 as a critical immune hub that positively modulates wheat resistance to leaf rust and is specifically targeted by the\n \n P.\u2009triticina<\/jats:italic>\n <\/jats:styled-content>\n effector Pt3863. Notably, Pt3863 has evolved a sophisticated virulence strategy to simultaneously disrupt both the phosphorylation\u2010mediated activation and proteasomal stability of TaRLCK176, thereby impairing host immune responses. This study elucidates a key molecular mechanism underlying the suppression of wheat immunity by\n \n P.\u2009triticina<\/jats:italic>\n <\/jats:styled-content>\n and highlights TaRLCK176 as a promising candidate target for the genetic engineering of durable resistance in wheat against leaf rust.\n <\/jats:p>","DOI":"10.1111\/mpp.70317","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T08:56:39Z","timestamp":1784537799000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Puccinia triticina<\/i>\n Effector Pt3863 Targets and Subverts\n TaRLCK176<\/scp>\n to Suppress Wheat Resistance to Leaf Rust"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4247-7925","authenticated-orcid":false,"given":"Jiaojie","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University Baoding 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