{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:16:55Z","timestamp":1787894215464,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pectobacterium parvum<\/jats:italic>\n                    is an emerging phytopathogen causing aerial stem rot of potato.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. parvum<\/jats:italic>\n                    <\/jats:styled-content>\n                    has a broad host range, with Chinese cabbage as one of its important hosts. Through multi\u2010omics analysis, we discovered a previously uncharacterized gene family highly expressed during\n                    <jats:italic>in planta<\/jats:italic>\n                    infection, designated the plant secondary metabolite\u2010binding (PSMB) family. This family likely originated via horizontal gene transfer and underwent lineage\u2010specific expansion in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. parvum<\/jats:italic>\n                    <\/jats:styled-content>\n                    , forming a four\u2010paralog pathogenicity island. Structural modelling revealed that PSMB proteins resemble the rhizobial RhiA protein, though their function in phytopathogens has not been established. Biochemical assays demonstrated that the representative member, PSMB1a, binds defensive plant secondary metabolites (PSMs) with high affinity. PSMB1a binds the solanidine with submicromolar affinity (K\n                    <jats:sub>d<\/jats:sub>\n                    \u2009=\u20090.103\u2009\u03bcM) and exhibited approximately 100\u2010fold higher affinity over salicylic acid (K\n                    <jats:sub>d<\/jats:sub>\n                    \u2009=\u20099.23\u2009\u00b1\u20092.35\u2009\u03bcM), indicating selectivity for specific PSMs. Functional studies demonstrated that PSMB1a enhances tolerance to defensive PSMs and contributes to virulence. Heterologous expression of\n                    <jats:italic>PSMB1a<\/jats:italic>\n                    in sister species\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. polare<\/jats:italic>\n                    <\/jats:styled-content>\n                    enhanced PSM tolerance and virulence on Chinese cabbage, but suppressed bacterial proliferation under non\u2010stress conditions. Conversely, deletion of the entire PSMB pathogenicity island in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. parvum<\/jats:italic>\n                    <\/jats:styled-content>\n                    attenuated virulence on potato stems, Chinese cabbage, and radish while increasing in\u00a0vitro growth, confirming a trade\u2010off between virulence contribution and basal fitness. These findings identify the PSMB family as a new class of virulence factors in\n                    <jats:italic>Pectobacterium<\/jats:italic>\n                    and reveal a specialized adaptive strategy in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. parvum<\/jats:italic>\n                    <\/jats:styled-content>\n                    that involves the binding of defensive PSMs to facilitate infection.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70328","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:52:06Z","timestamp":1787201526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Novel Plant Secondary Metabolite\u2010Binding Gene Family Required for Full Virulence in\n                    <i>Pectobacterium parvum<\/i>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4328-984X","authenticated-orcid":false,"given":"Jinhui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxing","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tangshan Academy of Agricultural Sciences  Tangshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingkui","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Horticulture and Forestry Science Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5249-563X","authenticated-orcid":false,"given":"Minna","family":"Pirhonen","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences University of Helsinki  Helsinki 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