{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:18:23Z","timestamp":1787894303315,"version":"build-2784847793"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31670650"],"award-info":[{"award-number":["31670650"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD0600103"],"award-info":[{"award-number":["2017YFD0600103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n The Phox and Bem1 (PB1) modular domain mediates specific protein\u2013protein interactions that are crucial for immune signalling in eukaryotes. However, the immune signalling network mediated by plant PB1 domain\u2010containing proteins remains poorly understood. In this study, we demonstrate that the poplar rust resistance proteins with PB1 domains, members 1 and 2 (PdRPM1 and PdRPM2), form heterodimers in poplar via their N\u2010terminal PB1 domains. These domains exhibit distinct amino acid sequences that diverge from the canonical PB1\u2013PB1 interactions found in other eukaryotes, representing a plant\u2010specific variant. Knockout of\n PdRPM1\/2<\/jats:italic>\n in poplar significantly increased both pustule number and total pustule area following infection with\n Melampsora larici\u2010populina<\/jats:italic>\n (Mlp). Further analysis revealed that\n PdRPM1\/2<\/jats:italic>\n contributes to poplar resistance against Mlp by regulating the levels of reactive oxygen species (ROS) and jasmonoyl\u2010isoleucine, the biosynthesis of flavonoids, as well as the transcription of defence\u2010related genes. Furthermore, loss of\n PdRPM1<\/jats:italic>\n was sufficient to increase poplar susceptibility to Mlp. We also identified a small secreted protein produced by Mlp during infection that interacts with PdRPM1 and disrupts the assembly of the PdRPM1\/2 complex. Based on its role, we named this protein\n M. larici\u2010populina<\/jats:italic>\n suppressor of dimerisation 1 (MlpSD1). Thus, we have identified a novel plant\u2010specific PB1 domain\u2010mediated disease resistance complex in poplar and uncovered a rust\u2010derived secreted protein that disrupted the formation of this complex.\n <\/jats:p>","DOI":"10.1111\/mpp.70329","type":"journal-article","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:43:08Z","timestamp":1787805788000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plant\u2010Specific\n PB1<\/scp>\n Domain Complex Mediates Poplar Defence and Is Targeted by Rust Fungus Secreted Protein\n MlpSD1<\/scp>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-854X","authenticated-orcid":false,"given":"Kexu","family":"Xin","sequence":"first","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingke","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changhao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9625-1239","authenticated-orcid":false,"given":"Zhongdong","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1266-5993","authenticated-orcid":false,"given":"Chunyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Forestry Northwest A&F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2025.112624"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv269"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22095001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13054"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019315108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01733"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19302"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.114.132753"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.672035"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.157370"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcv063"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.648253"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf642"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2024.06.010"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0831166100"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14786"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010702"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09185-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100400"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2015.04.007"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705147104"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-60733-9"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2019.09.010"},{"key":"e_1_2_9_25_1","unstructured":"R Core Team.2025.R: A Language and Environment for Statistical Computing. 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