{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:02:13Z","timestamp":1792267333960,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1401300"],"award-info":[{"award-number":["2022YFD1401300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2024JC\u2010YBQN\u20100165"],"award-info":[{"award-number":["2024JC\u2010YBQN\u20100165"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372602"],"award-info":[{"award-number":["32372602"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Oxysterol\u2010binding protein (OSBP)\u2010related proteins (ORPs) are indispensable eukaryotic lipid transporters and promising targets for pharmaceutical and agrochemical exploitation. Among ORP family members across kingdoms, oomycete ORP1 constitutes a phylogenetically distinct subtype that evolved independently in oomycetes, which is markedly divergent from ORP homologues in animals, plants and fungi. Oxathiapiprolin, the first commercial fungicide targeting OSBP family proteins, specifically acts on oomycete ORP1 and exhibits robust inhibitory efficacy against diverse\n                    <jats:italic>Phytophthora<\/jats:italic>\n                    pathogens and downy mildews. Nevertheless, the molecular function of oomycete ORP1 and the inhibitory mechanism of oxathiapiprolin remain poorly defined. Here, we demonstrate that PsORP1 of\n                    <jats:italic>Phytophthora sojae<\/jats:italic>\n                    specifically localises to ER\u2013Golgi membrane contact sites (MCSs) and is indispensable for vegetative growth and asexual\/sexual development of\n                    <jats:italic>P. sojae<\/jats:italic>\n                    . In\u00a0vitro liposome reconstitution assays confirmed that the conserved C\u2010terminal OSBP\u2010related domain (ORD) of PsORP1 mediates canonical phosphatidylserine (PS)\/phosphatidylinositol 4\u2010phosphate (PI4P) counter\u2010transport. Biochemical binding assays further verified that oxathiapiprolin directly interacts with the ORD of PsORP1 and competitively blocks PS\/PI4P lipid exchange. Collectively, this study demonstrates that PsORP1 localises to ER\u2013Golgi MCSs and mediates ORD\u2010dependent PS\/PI4P counter\u2010transport, which is essential for\n                    <jats:italic>P. sojae<\/jats:italic>\n                    development, and elucidates that oxathiapiprolin directly targets the ORD to competitively block this lipid exchange, providing a structural framework for rational design of next\u2010generation OSBP\u2010targeting inhibitors against oomycetes.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70331","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T04:55:40Z","timestamp":1788411340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The\n                    <scp>ER<\/scp>\n                    \u2013Golgi Lipid Exchanger\n                    <scp>PsORP1<\/scp>\n                    Is Essential for\n                    <i>Phytophthora sojae<\/i>\n                    Development and Is Blocked by Oxathiapiprolin via Its\n                    <scp>OSBP\u2010Related<\/scp>\n                    Domain (ORD)"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8407-5033","authenticated-orcid":false,"given":"Xiaofei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, College of Plant Protection China Agricultural University  Beijing China"},{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengcheng","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangda","family":"Shao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7710-4639","authenticated-orcid":false,"given":"Qin","family":"Peng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixia","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0564-5150","authenticated-orcid":false,"given":"Jianqiang","family":"Miao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7859-9741","authenticated-orcid":false,"given":"Xili","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, College of Plant Protection China Agricultural University  Beijing China"},{"name":"State Key Laboratory of Crop Stress Resistance and High\u2010Efficiency Production, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.funbio.2011.07.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/157.3.1117"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R111.338400"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01157"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsptsci.0c00207"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.625"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91345"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-024-01639-9"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aab1370"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2015.04.001"},{"key":"e_1_2_10_12_1","first-page":"2515","article-title":"Structural 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