{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:57Z","timestamp":1790575017611,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160036"],"award-info":[{"award-number":["32160036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360649"],"award-info":[{"award-number":["32360649"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Response regulators (RRs) of two\u2010component signalling systems (TCSs) containing tandem receiver (REC) domains are widespread in bacteria, yet their functions and regulatory mechanisms remain poorly understood. In our previous study, DrdR, one such RR in the cruciferous black rot disease pathogen\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Xanthomonas campestris<\/jats:italic>\n                    <\/jats:styled-content>\n                    pv.\n                    <jats:italic>campestris<\/jats:italic>\n                    (Xcc) was demonstrated to positively regulate pilus\u2010dependent motility and negatively regulate flagellum\u2010dependent motility. We showed that DrdR modulates the ATPase activities of pili motor proteins PilT and PilB, thereby enhancing bacterial pilus\u2010dependent swarming motility. However, how DrdR represses flagellar motility remained unknown. Here, we demonstrate that DrdR acts as a transcriptional repressor of flagellar gene expression. We used in\u00a0vitro and in\u00a0vivo approaches to identify FleQ, the master transcriptional regulator of flagellar genes, as a novel interaction partner of DrdR. Biochemical analyses revealed that DrdR binding inhibits FleQ's ATPase activity, which is essential for its transcriptional activation function. Microscale thermophoresis assays showed that DrdR reduces FleQ's DNA\u2010binding capability to its cognate promoter. These findings collectively indicate that DrdR modulates FleQ transcriptional activity by reducing both its DNA\u2010binding ability and ATPase activity. Our results demonstrate that DrdR serves as a specialized modulator of FleQ that acts upstream in the signalling cascade controlling the expression of flagellar genes in Xcc. This study exhibits a previously unknown mechanism whereby DrdR regulates bacterial motility. Combined with our previous finding, our data suggest that DrdR most likely acts as a conversion regulator between flagellum\u2010dependent and pilus\u2010dependent motility in Xcc.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70332","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:58:30Z","timestamp":1788328710000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>DrdR<\/scp>\n                    Negatively Modulates the Expression of Flagellar Genes via Interaction With\n                    <scp>FleQ<\/scp>\n                    in\n                    <scp>\n                      <i>Xanthomonas campestris<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5463-7298","authenticated-orcid":false,"given":"Pei\u2010Dong","family":"Ren","sequence":"first","affiliation":[{"name":"College of Life Science and Technology Guangxi University  Nanning China"},{"name":"Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Plant Protection Research Institute Guangxi Academy of Agricultural Science  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Ying","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Hua","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0891-1226","authenticated-orcid":false,"given":"Ji\u2010Liang","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology Guangxi University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6974-7266","authenticated-orcid":false,"given":"Rui\u2010Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Plant Protection Research Institute Guangxi Academy of Agricultural Science  Nanning 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