{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:57Z","timestamp":1790575017013,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171795"],"award-info":[{"award-number":["32171795"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Successful infection by plant\u2010pathogenic fungi requires both penetration of the host cuticle and tolerance of the reactive oxygen species (ROS) burst associated with host immunity. However, how these early infection events are temporally coordinated remains unclear. Here, we identified a three\u2010tier transcriptional cascade, ApHSF\u2013ApCtf1\u03b22\u2013ApCUT3, in\n                    <jats:italic>Arthrinium phaeospermum<\/jats:italic>\n                    , the causal agent of shoot blight in hybrid bamboo (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bambusa pervariabilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u00d7\n                    <jats:italic>grandis<\/jats:italic>\n                    ). Yeast one\u2010hybrid, electrophoretic mobility shift and dual\u2010luciferase assays showed that ApHSF directly binds the CTAGAA core motif in the\n                    <jats:italic>ApCtf1\u03b22<\/jats:italic>\n                    promoter and activates its transcription. Functional analyses further showed that ApHSF promotes detoxification of host\u2010derived ROS by activating the fungal antioxidant system, whereas pharmacological suppression of host ROS accumulation substantially restored the pathogenicity defect of the \u0394\n                    <jats:italic>ApHSF<\/jats:italic>\n                    mutant. ApHSF neither bound nor independently activated the\n                    <jats:italic>ApCUT3<\/jats:italic>\n                    promoter. Instead, yeast three\u2010hybrid and combinatorial dual\u2010luciferase assays showed that, under early oxidative stress, ApHSF acts as a cofactor to enhance ApCtf1\u03b22\u2010dependent activation of ApCUT3. In\u00a0vivo fluorescence imaging further demonstrated that ROS detoxification alone was insufficient for full pathogenicity, which additionally required an intact ApCtf1\u03b22\u2013ApCUT3 module for cuticle penetration and colonization. Phos\u2010tag immunoblotting, \u03bb\u2010protein phosphatase treatment and kinase inhibition assays showed that heat and oxidative stress induced phosphorylation of ApHSF and ApCtf1\u03b22 and that these phosphorylation responses depended on p38 MAPK activity. By contrast, host cuticle\u2010derived cues preferentially induced the ApCtf1\u03b22\u2013ApCUT3 module. Together, these findings reveal how a plant\u2010pathogenic fungus integrates oxidative and cuticle\u2010associated signals to coordinate early infection.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70334","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:58:01Z","timestamp":1788159481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>ApHSF<\/scp>\n                    \u2013\n                    <scp>ApCtf1\u03b22<\/scp>\n                    \u2013\n                    <scp>ApCUT3<\/scp>\n                    Cascade Couples Host\n                    <scp>ROS<\/scp>\n                    Detoxification to Cuticle Penetration During Fungal Pathogenesis"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0898-2979","authenticated-orcid":false,"given":"Peng","family":"Yan","sequence":"first","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5932-2993","authenticated-orcid":false,"given":"Sijia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3894-5398","authenticated-orcid":false,"given":"Zhanbo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"},{"name":"Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province  Chengdu China"},{"name":"Sichuan Mt. Emei Forest Ecosystem National Observation and Research Station  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0254-8075","authenticated-orcid":false,"given":"Han","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Forestry Sichuan Agricultural University  Chengdu China"},{"name":"Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province  Chengdu China"},{"name":"Sichuan Mt. 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