{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:57Z","timestamp":1790575017591,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802126"],"award-info":[{"award-number":["31802126"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Plant fungal pathogens secrete a plethora of effectors into host cells to facilitate their infection by interfering with the normal physiological processes of the host plant. However, the role of guanyl\u2010specific ribonuclease T1 effector protein in the virulence of fungal pathogens remains largely unexplored. Here, we show that an effector protein EC19, secreted by the soil\u2010borne fungus\n                    <jats:italic>Fusarium oxysporum<\/jats:italic>\n                    f. sp.\n                    <jats:italic>lycopersici<\/jats:italic>\n                    (Fol) that causes tomato wilt disease, contributes to the full virulence of Fol. In\u2010locus knockout of\n                    <jats:italic>EC19<\/jats:italic>\n                    significantly reduced the virulence of Fol, while complementing\n                    <jats:italic>EC19<\/jats:italic>\n                    in the knockout mutant restored the pathogenicity of Fol on tomato. EC19, which contains the ribonuclease T1\u2010like domain, is highly conserved across fungal species and exhibited the ability to cleave single\u2010stranded RNA isolated from tomato.\n                    <jats:italic>EC19<\/jats:italic>\n                    was specifically induced during Fol infection. The AlphaFold3\u2010predicted structure of EC19 shows a high degree of structural similarity with ribonuclease T1 from\n                    <jats:italic>Aspergillus oryzae<\/jats:italic>\n                    , with limited similarity to RNase T2. Using\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    \u2010mediated transient assay in the leaves of\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    , we found that EC19 predominantly localised to the nucleus of plant cells. Stable transgenic susceptible tomato plants overexpressing\n                    <jats:italic>EC19<\/jats:italic>\n                    exhibited increased susceptibility to Fol infection compared to wild\u2010type plants. RNA\u2010seq analysis of\n                    <jats:italic>EC19<\/jats:italic>\n                    \u2010overexpressing tomato lines revealed that downregulated differentially expressed genes were predominantly\u00a0enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, and plant\u2013pathogen interaction pathways. Taken together, these findings indicate that EC19 with ribonuclease activity promotes Fol infection, expanding our understanding of the molecular mechanisms underlying Fol virulence.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70335","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:42:18Z","timestamp":1788237738000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Conserved Guanyl\u2010Specific Ribonuclease\n                    <scp>T1<\/scp>\n                    Effector\n                    <scp>EC19<\/scp>\n                    Is Required for Full Virulence of the Vascular Wilt Fungus\n                    <i>Fusarium oxysporum<\/i>\n                    on Tomato"],"prefix":"10.1111","volume":"27","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, International Joint Laboratory for Molecular Breeding of Vegetables, College of Horticulture Hebei Agricultural University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3208-9224","authenticated-orcid":false,"given":"Yifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, International Joint Laboratory for Molecular Breeding of Vegetables, College of Horticulture Hebei Agricultural University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoqian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, International Joint Laboratory for Molecular Breeding of Vegetables, College of Horticulture Hebei Agricultural University  Baoding 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