{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:57Z","timestamp":1790575017585,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ5628"],"award-info":[{"award-number":["2024JJ5628"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Camellia oleifera<\/jats:italic>\n                    is an important woody oil crop in southern China, but its production is severely threatened by anthracnose caused by\n                    <jats:italic>Colletotrichum fructicola<\/jats:italic>\n                    .\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    deploys secreted effector proteins to establish infection. However, systematic identification and functional characterisation of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    effector genes upregulated during infection remains largely unexplored. Here, we integrated genome\u2010wide secretome prediction with RNA\u2010seq data from\n                    <jats:italic>C. oleifera<\/jats:italic>\n                    leaves inoculated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    to identify candidate effectors induced during infection, followed by functional screening, targeted gene deletion, complementation and pathogenicity assays. Five novel effectors required for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    full virulence were identified, all of which suppressed Bax\u2010induced cell death in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    . Targeted deletion of the corresponding genes in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    reduced lesion areas by 47%\u201378% on\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. oleifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves and by up to 67% on apple fruits, whereas complementation restored their virulence to wild\u2010type levels. Transcriptomic profiling of infected postharvest\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. oleifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    fruits identified differentially expressed genes enriched in GO terms related to copper ion response, as well as in KEGG pathways associated with phenylpropanoid biosynthesis, taurine and hypotaurine metabolism, and plant\u2013pathogen interactions. In addition, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities, as well as malondialdehyde content, were altered in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. oleifera<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves inoculated with the effector\u2010deletion mutants compared with those infected with the wild\u2010type strain, suggesting that these effectors may contribute to the modulation of host oxidative stress responses. Taken together, our findings provide genetic and physiological evidence that these effectors contribute to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. fructicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    virulence, offering potential targets for anthracnose control.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70338","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:32:07Z","timestamp":1788777127000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Systematic Identification and Functional Characterisation of\n                    <i>Colletotrichum fructicola<\/i>\n                    Effectors During\n                    <i>Camellia oleifera<\/i>\n                    Colonisation"],"prefix":"10.1111","volume":"27","author":[{"given":"Lingxue","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory for the Utilization of Woody Oil Resources Central South University of Forestry and Technology  Changsha China"},{"name":"Yuelushan Laboratory Central South University of Forestry and Technology  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentong","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory for the Utilization of Woody Oil Resources Central South University of Forestry and Technology  Changsha China"},{"name":"Yuelushan Laboratory Central South University of Forestry and Technology  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2178-994X","authenticated-orcid":false,"given":"Kaile","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Horticulture Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1807-2937","authenticated-orcid":false,"given":"Xizhe","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of North China Crop Improvement and Regulation, College of Horticulture Hebei Agricultural University  Baoding China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenxue","family":"Song","sequence":"additional","affiliation":[{"name":"College of Horticulture Henan Agricultural University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9288-869X","authenticated-orcid":false,"given":"Lisong","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of North China Crop Improvement and Regulation, College of Horticulture Hebei Agricultural University  Baoding 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