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Here, we identify a rice lesion mimic mutant,\n                    <jats:italic>osasa2\u20101<\/jats:italic>\n                    , which exhibits spontaneous cell death and enhanced disease resistance. The mutant displays white spots on leaves that develop into large brown lesions. Histological analysis reveals increased cell death and H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    accumulation. Map\u2010based cloning identified the causal gene as\n                    <jats:italic>OsASA2<\/jats:italic>\n                    (LOC_Os03g15780). The\n                    <jats:italic>osasa2\u20101<\/jats:italic>\n                    mutant carries a G496 deletion that introduces a premature stop codon, likely resulting in nonsense\u2010mediated decay of the mutant transcript. We demonstrate that OsASA2 interacts with OsASA1, OsASB1 and OsASB2. Its loss\u2010of\u2010function leads to hyperactivation of anthranilate synthase (AS) activity, enhancing tryptophan biosynthesis and metabolic flux into downstream pathways. The\n                    <jats:italic>osasa2\u20101<\/jats:italic>\n                    mutant exhibits elevated reactive oxygen species (ROS) levels, increased callose deposition and upregulation of defence\u2010related genes, contributing to enhanced resistance against both the rice blast fungus\n                    <jats:italic>Magnaporthe oryzae<\/jats:italic>\n                    and the bacterial blight pathogen\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Xanthomonas oryzae<\/jats:italic>\n                    <\/jats:styled-content>\n                    pv.\n                    <jats:italic>oryzae<\/jats:italic>\n                    . Our findings highlight a novel regulatory role for\n                    <jats:italic>OsASA2<\/jats:italic>\n                    in conferring broad\u2010spectrum disease resistance by modulating AS activity and the tryptophan biosynthetic pathway in rice.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70340","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:34:31Z","timestamp":1788777271000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>OsASA2<\/scp>\n                    Loss\u2010of\u2010Function Confers Broad\u2010Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice"],"prefix":"10.1111","volume":"27","author":[{"given":"Mingliang","family":"Guo","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute Institute of Crop Sciences (Fujian Germplasm Resources Center), Fujian Academy of Agricultural Sciences  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2734-7894","authenticated-orcid":false,"given":"Shuyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Agricultural Genetic Engineering, Biotechnology Research Institute Institute of Crop Sciences (Fujian Germplasm Resources Center), Fujian Academy of Agricultural Sciences  Fuzhou Fujian China"},{"name":"College of Agriculture, College of Life Sciences Fujian Agriculture and Forestry University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0894-3295","authenticated-orcid":false,"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"College of Agriculture, College of Life Sciences Fujian Agriculture and Forestry University  Fuzhou Fujian 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