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In this study, we investigated the molecular mechanisms underlying the efficient uptake of dsRNAs by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    , making significant progress towards addressing key gaps in our understanding of SIGS. We identified four\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    genes,\n                    <jats:italic>BcBMP1<\/jats:italic>\n                    ,\n                    <jats:italic>BcSOD1<\/jats:italic>\n                    ,\n                    <jats:italic>BcGAR2<\/jats:italic>\n                    , and\n                    <jats:italic>BcVELB<\/jats:italic>\n                    , as effective targets for grey mould control by employing an\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    <\/jats:styled-content>\n                    SIGS screening system. Furthermore, by constructing a T\u2010DNA insertion mutant library, we identified the \u0394\n                    <jats:italic>BcmfsR<\/jats:italic>\n                    mutant, which exhibited significantly impaired dsRNA internalization. Functional assays revealed that the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    Major Facilitator Superfamily protein, BcMFSR, is critical for pH\u2010dependent dsRNA uptake, facilitating SIGS efficacy. In the absence of functional BcMFSR, dsRNAs failed to reduce lesion size and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    virulence, regardless of the dsRNA sequence. BcMFSR is required for SIGS\u2010mediated silencing of four genes. Moreover, fluorescent labelling confirmed the localization of both BcMFSR and dsRNA in vesicular structures within hyphae, suggesting its involvement in endocytosis. Our discoveries not only established the novel role of BcMFSR in RNA uptake but also provided a foundation for improving the efficiency of RNA pesticides and broadening their agricultural applications.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70343","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:47:27Z","timestamp":1789973247000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>BcMFSR<\/scp>\n                    Is Required for Efficient Uptake of Exogenous\n                    <scp>dsRNA<\/scp>\n                    and Spray\u2010Induced Gene Silencing in\n                    <i>Botrytis cinerea<\/i>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0880-3691","authenticated-orcid":false,"given":"Le","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, 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