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However, whether cellulose\u2010binding proteins (CBPs), which are a class of CWMPs, can synergize with cellulases (a subgroup of CWDEs) to promote parasitism remains unclear. Here, we identified and characterized a CBP (\n                    <jats:italic>MgCBP1<\/jats:italic>\n                    ) and a \u03b2\u20101,4\u2010endoglucanase (\n                    <jats:italic>MgENG2<\/jats:italic>\n                    ) from the rice root\u2010knot nematode\n                    <jats:italic>Meloidogyne graminicola<\/jats:italic>\n                    . MgCBP1 contains a conserved cellulose\u2010binding module (CBM) and is specifically expressed in the subventral oesophageal glands, with transcript levels markedly up\u2010regulated in pre\u2010parasitic second\u2010stage juveniles (pre\u2010J2s) and during early infection. Recombinant MgCBP1 bound cellulose, disrupted fibre structure and enhanced cellulase\u2010mediated hydrolysis via its intact CBM, whereas a CBM\u2010disrupted mutant lack both activities. MgENG2, which lacks a CBM, exhibited strong endoglucanase activity and displayed an expression profile similar to that of\n                    <jats:italic>MgCBP1<\/jats:italic>\n                    . MgCBP1 significantly enhanced the hydrolytic efficiency of MgENG2, whereas its CBM\u2010disrupted mutant did not. Transgenic rice overexpressing\n                    <jats:italic>MgCBP1<\/jats:italic>\n                    showed enhanced susceptibility to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. graminicola<\/jats:italic>\n                    <\/jats:styled-content>\n                    . RNAi\u2010mediated individual silencing of\n                    <jats:italic>MgCBP1<\/jats:italic>\n                    or\n                    <jats:italic>MgENG2<\/jats:italic>\n                    both reduced infection, with\n                    <jats:italic>MgENG2<\/jats:italic>\n                    silencing showing a significantly greater effect. Co\u2010silencing of both genes yielded the greatest reduction in gall formation and parasitic nematode numbers, which was significantly greater than that of\n                    <jats:italic>MgCBP1<\/jats:italic>\n                    \u2010only silencing, but did not differ significantly from\n                    <jats:italic>MgENG2<\/jats:italic>\n                    \u2010only silencing. Collectively, these findings demonstrate that MgCBP1 promotes nematode parasitism by binding cellulose, disrupting fibril surface integrity and enhancing the cellulolytic efficiency of MgENG2, providing the first experimental evidence that a CBP from a plant pathogen can synergistically facilitate infection by boosting endoglucanase\u2010mediated cellulose degradation.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70346","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T03:16:04Z","timestamp":1789701364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>MgCBP1<\/scp>\n                    Synergistically Enhances\n                    <scp>MgENG2<\/scp>\n                    \u2010Mediated Cellulose Degradation to Promote Parasitism in\n                    <scp>\n                      <i>Meloidogyne graminicola<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"27","author":[{"given":"Chunhui","family":"Huang","sequence":"first","affiliation":[{"name":"College of Plant Protection South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Plant Protection South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Protection South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiani","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection South China Agricultural University  Guangzhou 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