{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:56:21Z","timestamp":1790740581810,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n The complete genome sequence of a relatively weak virulent tobacco pathogen,\n \n Pseudomonas syringae<\/jats:italic>\n <\/jats:styled-content>\n pv.\n tabaci<\/jats:italic>\n 8018 (\n Pta<\/jats:italic>\n 8018) was determined to elucidate key genetic elements that cause disease. Genome comparison of\n Pta<\/jats:italic>\n 8018 to the previously available\n P. syringae<\/jats:italic>\n pv.\n tabaci<\/jats:italic>\n strains revealed that\n Pta<\/jats:italic>\n 8018 and\n Pta<\/jats:italic>\n 11528 had high genome synteny, with an approximately 5\u2009Mb chromosome inversion in\n Pta<\/jats:italic>\n 6605. Furthermore,\n Pta<\/jats:italic>\n 8018 had lost the pathogenicity island GI\u20101 including a tabtoxin biosynthesis gene cluster and showed weak virulence on\n \n Nicotiana tabacum<\/jats:italic>\n <\/jats:styled-content>\n \u2018Xanthi NC\u2019 (Xanthi). When inoculated into several tobacco cultivars,\n Pta<\/jats:italic>\n 8018 caused leaf angular spot not only in Xanthi but also in\n \n N. tabacum<\/jats:italic>\n <\/jats:styled-content>\n cultivars Burley21 and N509, while\n Pta<\/jats:italic>\n 6605 did not cause disease in Burley21 and N509 due to the presence of avirulence factor HopAZ1. We found that a mutation in\n hopAZ1<\/jats:italic>\n allows\n Pta<\/jats:italic>\n 8018 to avoid recognition in these cultivars. Introduction of intact\n hopAZ1<\/jats:italic>\n of\n Pta<\/jats:italic>\n 6605 into\n Pta<\/jats:italic>\n 8018 rendered it avirulent to N509 and Burley21, revealing that effector HopAZ1 is a key virulence determinant of\n P. syringae<\/jats:italic>\n pv.\n tabaci<\/jats:italic>\n . Our data show that although\n Pta<\/jats:italic>\n 8018 has reduced virulence strength by the loss of GI\u20101 in Xanthi, it expanded its host range to two other cultivars through mutation of\n hopAZ1<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/mpp.70347","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:02:33Z","timestamp":1790737353000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["\n Pseudomonas syringae<\/i>\n <\/scp>\n pv.\n tabaci<\/i>\n 8018 is Virulent Against Tobacco Cultivars\n N509<\/scp>\n and Burley21 Due to Spontaneous Mutation in\n \n hopAZ1<\/i>\n <\/scp>"],"prefix":"10.1111","volume":"27","author":[{"given":"Yuto","family":"Hiraki","sequence":"first","affiliation":[{"name":"The Graduate School of Environmental, Life, Natural Science and Technology Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0941-6339","authenticated-orcid":false,"given":"Kana","family":"Kuroe","sequence":"additional","affiliation":[{"name":"The Graduate School of Environmental, Life, Natural Science and Technology Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3386-7180","authenticated-orcid":false,"given":"Hidenori","family":"Matsui","sequence":"additional","affiliation":[{"name":"The Graduate School of Environmental, Life, Natural Science and Technology Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-5554","authenticated-orcid":false,"given":"Motoki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Genomics and Breeding Iwate Biotechnology Research Center Iwate 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Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7767-7912","authenticated-orcid":false,"given":"Yuki","family":"Ichinose","sequence":"additional","affiliation":[{"name":"The Graduate School of Environmental, Life, Natural Science and Technology Okayama University Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.11.124"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.11.021"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020236\u20107"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/nar\/10.1.365","article-title":"Two\u2010Dimensional Graphic Analysis of DNA Sequence Homologies","volume":"10","author":"Harr R.","year":"1982","journal-title":"Nucleic 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