{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:56:57Z","timestamp":1790575017579,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","award":["KGM9942314"],"award-info":[{"award-number":["KGM9942314"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","award":["KGM5372322"],"award-info":[{"award-number":["KGM5372322"]}],"id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102020R1I1A2075335"],"award-info":[{"award-number":["NRF\u20102020R1I1A2075335"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The coat protein (CP) of turnip crinkle virus (TCV) plays multifaceted roles in viral infection, acting as both an avirulence (Avr) factor recognized by the resistance protein HRT and a viral suppressor of RNA silencing (VSR). However, how these dual activities are coordinated remains unclear. Here, we demonstrate that the position of a fluorescent protein (FP) tag on CP functionally separates its two activities. C\u2010terminal tagging (CP\u2010GFP) preserved the ability to trigger HRT\u2010dependent hypersensitive response (HR) but abolished silencing suppression, whereas N\u2010terminal tagging (YFP\u2010CP) retained VSR activity but failed to induce HR. These functional differences correlated with distinct subcellular localizations: Avr activity required both nuclear and cytoplasmic localization, while VSR function was confined to the cytoplasm. We further show that the N\u2010terminal region of CP associates with autophagy\u2010related protein 8 (ATG8), linking CP to autophagy\u2010associated antiviral pathways. Autophagy\u2010deficient\n                    <jats:italic>atg5<\/jats:italic>\n                    and\n                    <jats:italic>atg7<\/jats:italic>\n                    mutants exhibited enhanced susceptibility to TCV, supporting a role for autophagy in antiviral defence during infection. Together, our findings reveal that TCV CP contains spatially and mechanistically separable determinants for Avr and VSR functions and identify CP\u2013ATG8 association as a potential interface between viral infection and host autophagy\u2010associated regulation.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70350","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:32:15Z","timestamp":1790393535000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Dissection of the Turnip Crinkle Virus Coat Protein Links\n                    <scp>ATG8<\/scp>\n                    Association to Autophagy\u2010Associated Antiviral Regulation"],"prefix":"10.1111","volume":"27","author":[{"given":"Sung Hee","family":"Jo","sequence":"first","affiliation":[{"name":"Plant Systems Engineering Research Center KRIBB  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju Yeon","family":"Moon","sequence":"additional","affiliation":[{"name":"Plant Systems Engineering Research Center KRIBB  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukyoung","family":"Jung","sequence":"additional","affiliation":[{"name":"Plant Systems Engineering Research Center KRIBB  Daejeon Republic of Korea"},{"name":"Department of Life Sciences Korea University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1800-5929","authenticated-orcid":false,"given":"Gah\u2010Hyun","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Kentucky  Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4176-5096","authenticated-orcid":false,"given":"Pradeep","family":"Kachroo","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology University of Kentucky  Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3344-7875","authenticated-orcid":false,"given":"Jeong Mee","family":"Park","sequence":"additional","affiliation":[{"name":"Plant Systems Engineering Research Center KRIBB  Daejeon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1908710"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.11.5.781"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.107201"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1992.t01-24-00999.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2017.06.007"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.12.5.663"},{"key":"e_1_2_10_9_1","first-page":"1021","article-title":"Resistance and Susceptible Responses of \n                     Arabidopsis thaliana\n                   to Turnip Crinkle Virus","volume":"83","author":"Dempsey D. 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