{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:36:38Z","timestamp":1793255798743,"version":"4.5.0"},"reference-count":84,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272376"],"award-info":[{"award-number":["32272376"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Green mould is one of the major postharvest diseases affecting citrus. Although evidence indicates that microRNAs (miRNAs) participate in plant defence responses, their roles and regulatory mechanisms in citrus resistance to green mould remain unclear. Small RNA sequencing identified several defence\u2010associated miRNAs involved in citrus resistance to\n                    <jats:italic>Penicillium digitatum<\/jats:italic>\n                    . Among them, transient overexpression screening identified Csi\u2010miR3954b as the most effective miRNA, reducing disease incidence by 24.4% compared with the control at 5\u2009days post\u2010inoculation (dpi). Subsequent transient overexpression and short tandem target mimic (STTM)\u2010mediated suppression assays demonstrated that Csi\u2010miR3954b positively regulates citrus resistance to green mould, with transient overexpression reducing disease incidence by 22.7% and STTM\u2010mediated suppression increasing it by 9.6% at 5 dpi. Degradome sequencing, transient assays in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    leaves and expression analysis identified\n                    <jats:italic>CsCE70<\/jats:italic>\n                    and\n                    <jats:italic>CsPLA1<\/jats:italic>\n                    as the direct targets of Csi\u2010miR3954b. Transcriptome analysis showed that\n                    <jats:italic>CsCE70<\/jats:italic>\n                    and\n                    <jats:italic>CsPLA1<\/jats:italic>\n                    mainly regulated phenolic acid and flavonoid biosynthesis, respectively. Transient overexpression of\n                    <jats:italic>CsCE70<\/jats:italic>\n                    and\n                    <jats:italic>CsPLA1<\/jats:italic>\n                    increased disease incidence by 17.4% and 10.4%, respectively, compared with the control at 5 dpi, suggesting that both genes negatively regulated citrus resistance to\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">P. digitatum<\/jats:styled-content>\n                      .\n                    <\/jats:italic>\n                    Silencing these genes enhanced its resistance, accompanied by the accumulation of phenolics and flavonoids in the peel. In general, the results showed that the Csi\u2010miR3954b\u2013\n                    <jats:italic>CsCE70\/CsPLA1<\/jats:italic>\n                    regulatory modules enhanced citrus resistance to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. digitatum<\/jats:italic>\n                    <\/jats:styled-content>\n                    by promoting the biosynthesis of phenolic acids and flavonoids. These results provide new insights into miRNA\u2010mediated postharvest disease resistance and potential molecular strategies for improving citrus postharvest protection.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70351","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:11:43Z","timestamp":1791007903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Csi\u2010\n                    <scp>miR3954b<\/scp>\n                    \u2010\n                    <i>CsCE70\/CsPLA1<\/i>\n                    Regulatory Module Enhances Citrus Fruit Resistance to\n                    <i>Penicillium digitatum<\/i>\n                    by Promoting Phenolic Acid and Flavonoid Biosynthesis"],"prefix":"10.1111","volume":"27","author":[{"given":"Jixin","family":"Tian","sequence":"first","affiliation":[{"name":"College of Food Science Southwest University  Chongqing 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