{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:56:29Z","timestamp":1790740589119,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Viral infection often induces chlorosis, a symptom closely associated with chlorophyll (Chl) degradation. STAY\u2010GREEN (SGR) proteins are key regulators of Chl breakdown, but their roles in plant virus resistance remain poorly understood. In this study, we identified six\n                    <jats:italic>NtSGR<\/jats:italic>\n                    family genes in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    <\/jats:styled-content>\n                    and functionally characterized\n                    <jats:italic>NtSGRL1<\/jats:italic>\n                    during tomato yellow leaf curl virus (TYLCV) infection. TYLCV drastically upregulates\n                    <jats:italic>NtSGRL1<\/jats:italic>\n                    (149\u2010fold at 4 days post\u2010inoculation [dpi] vs. control), suggesting its involvement in the tobacco\u2013TYLCV interaction. Subcellular localization revealed that NtSGRL1 is targeted to both the nucleus and chloroplast, and yeast assays confirmed its transcriptional activation activity, indicating potential dual functions. TYLCV accumulation was reduced by 1995\u2010fold in\n                    <jats:italic>Ntsgrl1<\/jats:italic>\n                    mutants relative to wild\u2010type (WT) plants at 10 dpi. Further assays with tomato spotted wilt virus (TSWV), potato virus Y (PVY) and tobacco mosaic virus (TMV) showed that viral accumulation in\n                    <jats:italic>Ntsgrl1<\/jats:italic>\n                    mutants was reduced by 62%\u201399%, suggesting that NtSGRL1 may affect resistance to additional tested viruses. Transcriptomics revealed 1470 differentially expressed genes in TYLCV\u2010infected\n                    <jats:italic>Ntsgrl1<\/jats:italic>\n                    plants enriched in MAPK signalling and phenylpropanoid biosynthesis pathways. Virus\u2010induced gene silencing (VIGS)\u2010based validation further supported the involvement of these pathways in TYLCV resistance. Overall, this study identifies\n                    <jats:italic>NtSGRL1<\/jats:italic>\n                    as a critical regulator of antiviral resistance in tobacco and reveals its dual functional roles as both a Chl\u2010related enzyme and a transcriptional regulator. These findings provide new insights into plant antiviral mechanisms and potential genetic resources for breeding virus\u2010resistant crops.\n                  <\/jats:p>","DOI":"10.1111\/mpp.70356","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:08:13Z","timestamp":1790737693000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genome\u2010Wide Screening and Functional Verification of the Tobacco\n                    <scp>SGR<\/scp>\n                    Family Identify Transcription\u2010Factor\u2010Like\n                    <scp>NtSGRL<\/scp>\n                    as a Negative Regulator of Tomato Yellow Leaf Curl Virus Resistance"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6534-7772","authenticated-orcid":false,"given":"Ming","family":"Gu","sequence":"first","affiliation":[{"name":"Liaoning Key Laboratory of Plant Pathology, 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