{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:47:12Z","timestamp":1791877632300,"version":"4.3.4"},"reference-count":56,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32001856"],"award-info":[{"award-number":["32001856"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871913"],"award-info":[{"award-number":["31871913"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Botrytis cinerea<\/jats:italic>\n triggers destructive grey mould with substantial economic losses, yet the functions of numerous pathogenicity genes remain largely unclear. Here, by screening a T\u2010DNA insertion library, we identified\n BcARS2<\/jats:italic>\n , a key component of the Microprocessor complex.\n BcARS2<\/jats:italic>\n is constitutively expressed, localizes to the nucleus, and modulates gene expression. The \u0394\n Bcars2<\/jats:italic>\n mutants exhibited impaired vegetative growth, enhanced conidiophore sporulation, increased conidiation, reduced sclerotial size, and increased sensitivity to multiple stresses. Pathogenicity assays showed that different inoculation methods led to distinct virulence phenotypes in \u0394\n Bcars2<\/jats:italic>\n mutants. Microscopic observations revealed that although the \u0394\n Bcars2<\/jats:italic>\n mutant formed normal appressoria and successfully invaded host cells, its invasive hyphae exhibited limited expansion. Furthermore, exogenous cAMP did not restore the impaired development of infection cushions (ICs), resulting in defective infection. Multitranscriptome analyses demonstrated that\n BcARS2<\/jats:italic>\n positively regulates\n BcPDI1<\/jats:italic>\n and\n BcCGF1<\/jats:italic>\n , two key genes responsible for reactive oxygen species (ROS) homeostasis and highly expressed during the IC developmental stage. Correspondingly, deletion of\n BcARS2<\/jats:italic>\n upregulated catalase gene expression, elevated catalase activity, reduced H\n 2<\/jats:sub>\n O\n 2<\/jats:sub>\n accumulation, disturbed ROS balance, and ultimately inhibited IC formation. In addition, most toxin biosynthesis\u2010related genes, which are normally upregulated during IC development, were significantly downregulated in the \u0394\n Bcars2<\/jats:italic>\n mutant. Collectively,\n BcARS2<\/jats:italic>\n modulates the virulence of\n \n B. cinerea<\/jats:italic>\n <\/jats:styled-content>\n by regulating IC development, vegetative hyphal growth, and stress responses.\n <\/jats:p>","DOI":"10.1111\/mpp.70359","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:04:49Z","timestamp":1791875089000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Arsenic Resistance Protein\n BcArs2<\/scp>\n Modulates Endogenous\n ROS<\/scp>\n Homeostasis in Infection Cushions to Enhance the Virulence of\n Botrytis cinerea<\/i>\n Toward Host Plants"],"prefix":"10.1111","volume":"27","author":[{"given":"Yinshan","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiang","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7240-4616","authenticated-orcid":false,"given":"Guihua","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2758-4146","authenticated-orcid":false,"given":"Mingzhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Ming","family":"Qin","sequence":"additional","affiliation":[{"name":"College of Plant Science Jilin University Changchun China"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine, Christopher S. Bond Life Sciences Center The University of Missouri Columbia Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2016.09.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102010\u201011\u201010\u2010r106"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2703"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02326"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar4142"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.14082"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.09.010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201033905\u2010x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.15416"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364\u20103703.2010.00692.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3711"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364\u20103703.2012.00822.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.13613"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-23371-0","volume-title":"Botrytis\u2014The Fungus, the Pathogen and Its Management in Agricultural Systems","author":"Fillinger S.","year":"2016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102958.2003.03866.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.04.046"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/brv.12057"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307969100"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010023\u201040093\u20104"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.16200"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010018\u20100301\u2010z"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08467"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2018.6777"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403115101"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0802493105"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12788"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400806"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.01042\u201022"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00960"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.068692.118"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294\u2010019\u201000952\u20104"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2016.10.003"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.13.6.1263"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1204915109"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/20.2.311"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074740"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.13404"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21572"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad251"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS\u201094\u20105\u20100551"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22041694"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof11010016"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12091710"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2017.1291112"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2016.151"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1239705"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.048637"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u2010313X.2006.02835.x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804218105"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108386"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12934"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof11060429"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.4c03744"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2019.103299"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/35044005"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2023.135443"}],"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.70359","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mpp.70359","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mpp.70359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:04:51Z","timestamp":1791875091000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1111\/mpp.70359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":56,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/mpp.70359"],"URL":"https:\/\/doi.org\/10.1111\/mpp.70359","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70359"}}