{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:10:30Z","timestamp":1771632630851,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T00:00:00Z","timestamp":1409270400000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"Abstract<\/jats:title>\n \n A study to determine the most appropriate antigen for use in the serodiagnosis of\n Cephenemyia<\/jats:italic>\n (\n D<\/jats:styled-content>\n iptera:\n O<\/jats:styled-content>\n estridae) infestation in roe deer (\n Capreolus capreolus<\/jats:italic>\n ) was carried out using immunoenzymatic tests. Serum samples from 43 roe deer from northern\n S<\/jats:styled-content>\n pain were obtained post\u2010mortem and corresponding numbers of bot fly larvae established. Three antigen complexes were tested, including\n Cephenemyia stimulator<\/jats:italic>\n C<\/jats:styled-content>\n lark excretory\/secretory antigens (\n CsES<\/jats:styled-content>\n ),\n C.\u2009stimulator<\/jats:italic>\n somatic antigens (\n CsSA<\/jats:styled-content>\n ) and\n Oestrus ovis<\/jats:italic>\n L.<\/jats:styled-content>\n (\n D<\/jats:styled-content>\n iptera:\n O<\/jats:styled-content>\n estridae) excretory\/secretory antigens (\n OoES<\/jats:styled-content>\n ). In addition, the composition of each antigen was analysed using an electrophoresis system.\n Cephenemyia stimulator<\/jats:italic>\n larvae were found in 25% of roe deer; the mean intensity of infection was 24.3 larvae per infested animal. In the antigen analysis,\n CsSA<\/jats:styled-content>\n showed four exclusive bands of molecular weight (17\u201319, 62, 65 and 67\u201370\u2009kDa). A positive correlation between immunoglobulin G (\n IgG<\/jats:styled-content>\n ) values and total number of larvae was found with\n CsES<\/jats:styled-content>\n and\n CsSA<\/jats:styled-content>\n . The highest sensitivity value, negative predictive value and negative likelihood ratio were obtained using\n CsES<\/jats:styled-content>\n . The highest specificity value, positive likelihood ratio and kappa value were achieved with\n CsSA<\/jats:styled-content>\n . The predictive values of the enzyme\u2010linked immunosorbent assay (\n ELISA<\/jats:styled-content>\n ) using\n CsES<\/jats:styled-content>\n and\n CsSA<\/jats:styled-content>\n reached statistical significance and seroprevalence values were 26\u201344%. The use of\n ELISA<\/jats:styled-content>\n with\n CsES<\/jats:styled-content>\n and\n CsSA<\/jats:styled-content>\n seems promising in the non\u2010invasive diagnosis of\n Cephenemyia<\/jats:italic>\n infestation in roe deer.\n <\/jats:p>","DOI":"10.1111\/mve.12080","type":"journal-article","created":{"date-parts":[[2014,8,29]],"date-time":"2014-08-29T05:24:09Z","timestamp":1409289849000},"page":"83-89","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Antigen characterization from second instars of oestrid bot flies for the detection of anti\u2010\n Cephenemyia stimulator<\/i>\n antibodies by\n ELISA<\/scp>\n in roe deer (\n Capreolus capreolus<\/i>\n )"],"prefix":"10.1111","volume":"28","author":[{"given":"M. S.","family":"ARIAS","sequence":"first","affiliation":[{"name":"Parasitology and Parasitic Diseases, Department of Animal Pathology Faculty of Veterinary Science, Santiago de Compostela University Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"PAJARES","sequence":"additional","affiliation":[{"name":"Parasitology and Parasitic Diseases, Department of Animal Pathology Faculty of Veterinary Science, Santiago de Compostela University Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"PAZ\u2010SILVA","sequence":"additional","affiliation":[{"name":"Parasitology and Parasitic Diseases, Department of Animal Pathology Faculty of Veterinary Science, Santiago de Compostela University Lugo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"D\u00cdEZ\u2010BA\u00d1OS","sequence":"additional","affiliation":[{"name":"Department of Animal Health Faculty of Veterinary Science, University of Le\u00f3n Le\u00f3n Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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