{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:35:57Z","timestamp":1773286557336,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T00:00:00Z","timestamp":1432598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Abstract<\/jats:title>\n \n Animal models have been developed for the study of rickettsial pathogenesis. However, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. To address this, we analysed the role of tick saliva in the transmission of\n \n Rickettsia conorii<\/jats:italic>\n <\/jats:styled-content>\n (\n R<\/jats:styled-content>\n ickettsiales:\n R<\/jats:styled-content>\n ickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible\n C3H<\/jats:styled-content>\n \/\n HeJ<\/jats:styled-content>\n mice with this\n \n Rickettsia<\/jats:italic>\n <\/jats:styled-content>\n , and infesting one group with nymphal\n \n Rhipicephalus sanguineus<\/jats:italic>\n <\/jats:styled-content>\n sensu lato<\/jats:italic>\n (\n I<\/jats:styled-content>\n xodida:\n I<\/jats:styled-content>\n xodidae) ticks. Quantification of bacterial loads and\n mRNA<\/jats:styled-content>\n levels of interleukin\u20101\u03b2 (\n IL<\/jats:styled-content>\n \u20101\u03b2),\n IL<\/jats:styled-content>\n \u201010 and\n NF<\/jats:styled-content>\n \u2010\u03baB was performed in\n C3H<\/jats:styled-content>\n \/\n HeJ<\/jats:styled-content>\n lung samples by real\u2010time quantitative polymerase chain reaction (\n PCR<\/jats:styled-content>\n ) and real\u2010time reverse transcriptase\n PCR<\/jats:styled-content>\n , respectively. Lung histology was examined to evaluate the pathological manifestations of infection. No statistically significant difference in bacterial load in the lungs of mice was observed between these two groups; however, a statistically significant difference was observed in levels of\n IL<\/jats:styled-content>\n \u20101\u03b2 and\n NF<\/jats:styled-content>\n \u2010\u03baB, both of which were higher in the group inoculated with rickettsiae but not infected with ticks. Lung histology in both groups of animals revealed infiltration of inflammatory cells. Overall, this study showed that i.d. inoculation of\n \n R.\u2009conorii<\/jats:italic>\n <\/jats:styled-content>\n caused infection in the lungs of\n C3H<\/jats:styled-content>\n \/\n HeJ<\/jats:styled-content>\n mice and tick saliva inhibited proinflammatory effects.\n <\/jats:p>","DOI":"10.1111\/mve.12118","type":"journal-article","created":{"date-parts":[[2015,5,26]],"date-time":"2015-05-26T05:34:11Z","timestamp":1432618451000},"page":"225-229","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The role of\n \n R<\/scp>\n hipicephalus sanguineus sensu lato\n <\/i>\n saliva in the dissemination of\n \n R<\/scp>\n ickettsia conorii\n <\/i>\n in\n C3H<\/scp>\n \/\n HeJ<\/scp>\n mice"],"prefix":"10.1111","volume":"29","author":[{"given":"N.","family":"MILHANO","sequence":"first","affiliation":[{"name":"Centre for the Study of Vectors and Infectious Diseases Dr Francisco Cambournac National Institute of Health Dr Ricardo Jorge \u00c1guas de Moura Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. B.","family":"SAITO","sequence":"additional","affiliation":[{"name":"Department of Pathology Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch Galveston TX U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"BECHELLI","sequence":"additional","affiliation":[{"name":"Department of Pathology Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch Galveston TX U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"FANG","sequence":"additional","affiliation":[{"name":"Department of Pathology Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch Galveston TX U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"VILHENA","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of \u00c9vora \u00c9vora Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"DE SOUSA","sequence":"additional","affiliation":[{"name":"Centre for the Study of Vectors and Infectious Diseases Dr Francisco Cambournac National Institute of Health Dr Ricardo Jorge \u00c1guas de Moura Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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