{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:49:03Z","timestamp":1784893743798,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","award":["88881.030429\/2013-01"],"award-info":[{"award-number":["88881.030429\/2013-01"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The mosquito\n                    <jats:italic>Stegomyia aegypti<\/jats:italic>\n                    (=\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    ) (\n                    <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                    iptera:\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    ulicidae) is a vector for the dengue and yellow fever viruses. As blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. The effects of the insecticide imidacloprid on the survival of\n                    <jats:italic>St.\u2009aegypti<\/jats:italic>\n                    were investigated and the sub\u2010lethal effects of the insecticide on midgut development were determined. Third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 and 15.0\u2009p.p.m.) and survival was monitored every 24\u2009h for 10\u2009days. Midguts from imidacloprid\u2010treated insects at different stages of development were dissected and processed for analyses by transmission electron microscopy, immunofluorescence microscopy and terminal deoxynucleotidyl transferase\n                    <jats:styled-content style=\"fixed-case\">dUTP<\/jats:styled-content>\n                    nick\u2010end labelling (\n                    <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content>\n                    ) assays. Imidacloprid concentrations of 3.0 and 15.0\u2009p.p.m. were found to affect midgut development similarly. Digestive cells of the fourth instar larvae (\n                    <jats:styled-content style=\"fixed-case\">L4<\/jats:styled-content>\n                    ) midgut exposed to imidacloprid had more multilamellar bodies, abundantly found in the cell apex, and more electron\u2010lucent vacuoles in the basal region compared with those from untreated insects. Moreover, imidacloprid interfered with the differentiation of regenerative cells, dramatically reducing the number of digestive and endocrine cells and leading to malformation of the midgut epithelium in adults. The data demonstrate that imidacloprid can reduce the survival of mosquitoes and thus indicate its potentially high efficacy in the control of\n                    <jats:italic>St.\u2009aegypti<\/jats:italic>\n                    populations.\n                  <\/jats:p>","DOI":"10.1111\/mve.12122","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T05:48:43Z","timestamp":1431496123000},"page":"245-254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Imidacloprid impairs the post\u2010embryonic development of the midgut in the yellow fever mosquito\n                    <i>\n                      <scp>S<\/scp>\n                      tegomyia aegypti\n                    <\/i>\n                    (=\n                    <i>\n                      <scp>A<\/scp>\n                      edes aegypti\n                    <\/i>\n                    )"],"prefix":"10.1111","volume":"29","author":[{"given":"K. M.","family":"FERNANDES","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. G.","family":"GONZAGA","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. V.","family":"PASCINI","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. R.","family":"MIRANDA","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. V. V.","family":"TOM\u00c9","sequence":"additional","affiliation":[{"name":"Departamento de Entomologia Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"SERR\u00c3O","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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