{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:07:20Z","timestamp":1766869640072,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T00:00:00Z","timestamp":1460505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>\n \n Mosquitoes are vectors for pathogens of malaria, lymphatic filariasis, dengue, chikungunya, yellow fever and\n J<\/jats:styled-content>\n apanese encephalitis.\n \n C<\/jats:styled-content>\n ulex quinquefasciatus\n <\/jats:italic>\n S<\/jats:styled-content>\n ay, 1823 (\n D<\/jats:styled-content>\n iptera:\n C<\/jats:styled-content>\n ulicidae) is a known vector of lymphatic filariasis. Its control in Brazil has been managed using the organophosphate temephos. Studies examining the proteins of\n \n C<\/jats:styled-content>\n x. quinquefasciatus\n <\/jats:italic>\n that are differentially expressed in response to temephos further understanding of the modes of action of the insecticide and may potentially identify resistance factors in the mosquito. In the present study, a comparative proteomic analysis, using 2\u2010dimensional electrophoresis coupled with matrix\u2010assisted laser desorption\/ionization (\n MALDI<\/jats:styled-content>\n ) time of flight (\n TOF<\/jats:styled-content>\n )\/\n TOF<\/jats:styled-content>\n mass spectrometry, and bioinformatics analyses were performed to identify midgut proteins in\n \n C<\/jats:styled-content>\n x. quinquefasciatus\n <\/jats:italic>\n larvae that were differentially expressed in response to exposure to temephos relative to those in untreated controls. A total of 91 protein spots were differentially expressed; 40 were upregulated and 51 were downregulated by temephos. A total of 22 proteins, predominantly upregulated, were identified as known to play a role in the immune response, whereas the downregulated proteins were involved in energy and protein catabolism. This is the first proteome study of the midgut of\n \n C<\/jats:styled-content>\n x. quinquefasciatus\n <\/jats:italic>\n and it provides insights into the molecular mechanisms of insecticide\u2010induced responses in the mosquito.\n <\/jats:p>","DOI":"10.1111\/mve.12172","type":"journal-article","created":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T03:10:31Z","timestamp":1460517031000},"page":"253-263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential protein expression in the midgut of\n \n C<\/scp>\n ulex quinquefasciatus\n <\/i>\n mosquitoes induced by the insecticide temephos"],"prefix":"10.1111","volume":"30","author":[{"given":"P. D.","family":"GAMES","sequence":"first","affiliation":[{"name":"Department of General Biology State University of Vi\u00e7osa Vi\u00e7osa Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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WHO report 818.WHO Geneva."},{"key":"e_1_2_6_41_1","unstructured":"World Health Organization(2006)Pesticides and their application. 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