{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:25:34Z","timestamp":1762323934629,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T00:00:00Z","timestamp":1461715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro [FAPERJ (Foundation for Support to Research in the State of Rio de Janeiro)]","award":["APQ1 E-26\/111.719\/2012"],"award-info":[{"award-number":["APQ1 E-26\/111.719\/2012"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico [CNPq (National Council of Technological and Scientific Development)]","award":["Universal 481843\/2013-2"],"award-info":[{"award-number":["Universal 481843\/2013-2"]}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"Abstract<\/jats:title>\n \n Amblyomma sculptum<\/jats:italic>\n (\n I<\/jats:styled-content>\n xodida:\n I<\/jats:styled-content>\n xodidae)\n B<\/jats:styled-content>\n erlese, 1888, a member of the\n \n A<\/jats:styled-content>\n mblyomma cajennense\n <\/jats:italic>\n complex, is the major vector of\n B<\/jats:styled-content>\n razilian spotted fever (\n BSF<\/jats:styled-content>\n ) in southeastern\n B<\/jats:styled-content>\n razil. In this study, the genetic diversity of\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n populations in the state of\n R<\/jats:styled-content>\n io\n de J<\/jats:styled-content>\n aneiro (\n RJ<\/jats:styled-content>\n ),\n B<\/jats:styled-content>\n razil, was investigated because genetic variability in tick populations may be related to vector competence. Samples of\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n from 19 municipalities in 7 regions of\n RJ<\/jats:styled-content>\n were subjected to\n DNA<\/jats:styled-content>\n extraction, amplification and sequencing of\n D<\/jats:styled-content>\n \u2010loop, cytochrome oxidase\n II<\/jats:styled-content>\n and\n 12S rDNA<\/jats:styled-content>\n mitochondrial genes. These sequences were used to map the genetic diversity of this tick.\n \n A<\/jats:styled-content>\n mblyomma sculptum\n <\/jats:italic>\n populations are genetically diverse in\n RJ<\/jats:styled-content>\n , especially in the\n S<\/jats:styled-content>\n outh\n C<\/jats:styled-content>\n entre and\n H<\/jats:styled-content>\n ighland regions. Few unique haplotypes were observed in all populations, and the majority of genetic variation found was among ticks within each population. Phylogenetic reconstruction reinforced the assumption that all the haplotypes identified in\n RJ<\/jats:styled-content>\n belong to\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n . However, some\n RJ<\/jats:styled-content>\n haplotypes are closer to\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n from\n A<\/jats:styled-content>\n rgentina than to\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n from elsewhere in\n B<\/jats:styled-content>\n razil. In\n RJ<\/jats:styled-content>\n ,\n \n A.<\/jats:styled-content>\n \u2009sculptum\n <\/jats:italic>\n has high genetic diversity, although little genetic differentiation. Observations also indicated a high level of gene flow among the studied populations and no evidence of population structure according to region in\n RJ<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/mve.12174","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T13:29:05Z","timestamp":1461763745000},"page":"342-350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Analysis of\n \n A<\/scp>\n mblyomma sculptum\n <\/i>\n haplotypes in an area endemic for\n B<\/scp>\n razilian spotted fever"],"prefix":"10.1111","volume":"30","author":[{"given":"K.","family":"BITENCOURTH","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro RJ Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biodiversidade e Sa\u00fade Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"VOLOCH","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Evolutiva Te\u00f3rica e Aplicada Universidade Federal do Rio de Janeiro Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"SERRA\u2010FREIRE","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro RJ Brazil"},{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Biodiversidade e Sa\u00fade Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"MACHADO\u2010FERREIRA","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular de Eucariontes e Simbiontes Universidade Federal do Rio de Janeiro Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"AMORIM","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Refer\u00eancia Nacional em Vetores das Riquetsioses Funda\u00e7\u00e3o Oswaldo Cruz Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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