{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T12:14:10Z","timestamp":1774700050238,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T00:00:00Z","timestamp":1465430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["PTDC\/SAU-SAP\/113523\/2009"],"award-info":[{"award-number":["PTDC\/SAU-SAP\/113523\/2009"]}]},{"name":"Global Health and Tropical Medicine","award":["UID\/Multi\/04413\/2013"],"award-info":[{"award-number":["UID\/Multi\/04413\/2013"]}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Wolbachia pipientis<\/jats:italic>\n                    (Rickettsiales: Rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. However, the effect of its co\u2010infection on vector competence for\n                    <jats:italic>Dirofilaria immitis<\/jats:italic>\n                    (Spirurida: Onchocercidae) in the wild has not been investigated. This study aimed to screen vectors of\n                    <jats:italic>D. immitis<\/jats:italic>\n                    for\n                    <jats:italic>w<\/jats:italic>\n                    Pip, to characterize these, and to investigate a possible association between the occurrence of\n                    <jats:italic>W. pipientis<\/jats:italic>\n                    and that of the nematode. The presence of\n                    <jats:italic>W. pipientis<\/jats:italic>\n                    was assessed in the five mosquito potential vectors of\n                    <jats:italic>D. immitis<\/jats:italic>\n                    in Portugal. Polymerase chain reaction (PCR) products were sequenced, and\n                    <jats:italic>w<\/jats:italic>\n                    Pip haplotypes were determined by PCR\u2013restricted fragment length polymorphism (RFLP). Results showed that\n                    <jats:italic>w<\/jats:italic>\n                    Pip was detected in 61.5% of\n                    <jats:italic>Culex pipiens<\/jats:italic>\n                    (Diptera: Culicidae) pools and 6.3% of\n                    <jats:italic>Culex theileri<\/jats:italic>\n                    pools.\n                    <jats:italic>w<\/jats:italic>\n                    Pip 16s rRNA sequences found in\n                    <jats:italic>Cx. theileri<\/jats:italic>\n                    exactly match those from\n                    <jats:italic>Cx. pipiens<\/jats:italic>\n                    , confirming a mosquito origin, rather than a nematode origin, as some specimens were infected with\n                    <jats:italic>D. immitis<\/jats:italic>\n                    . Only\n                    <jats:italic>w<\/jats:italic>\n                    Pip haplotype I was found. No association was found between the presence of\n                    <jats:italic>w<\/jats:italic>\n                    Pip and\n                    <jats:italic>D. immitis<\/jats:italic>\n                    in mosquitoes and hence a role for this endosymbiont in influencing vectorial competence is yet to be identified. This study contributes to understanding of\n                    <jats:italic>w<\/jats:italic>\n                    Pip distribution in mosquito populations and, to the best of the authors' knowledge, is the first report of natural infections by\n                    <jats:italic>w<\/jats:italic>\n                    Pip in\n                    <jats:italic>Cx. theileri<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mve.12179","type":"journal-article","created":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T03:58:04Z","timestamp":1465444684000},"page":"301-309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular detection of\n                    <i>Wolbachia pipientis<\/i>\n                    in natural populations of mosquito vectors of\n                    <i>Dirofilaria immitis<\/i>\n                    from continental Portugal: first detection in\n                    <i>Culex theileri<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"V.","family":"DE PINHO MIX\u00c3O","sequence":"first","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"MENDES","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"},{"name":"Escola Superior de Tecnologia da Sa\u00fade de Lisboa, Instituto Polit\u00e9cnico de Lisboa  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. L.","family":"MAUR\u00cdCIO","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"CALADO","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. T.","family":"NOVO","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"BELO","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine, GHTM, UEI Medical Parasitology, Instituto de Higiene e Medicina Tropical, IHMT Universidade Nova de Lisboa, UNL  Lisbon Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P. 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